Mutation in the bimD gene of Aspergillus nidulans confers a conditional mitotic block and sensitivity to DNA damaging agents

Journal Article · Sun Aug 01 00:00:00 EDT 1993 · Genetics; (United States)

OSTI ID:5425279

Denison, S H; May, G S ^[1]; Kaefer, E ^[2]

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1. Baylor College of Medicine, Houston, TX (United States)
2. Simon Frazer Univ., Burnaby, British Columbia (Canada)

Mutation in the bimD gene of Aspergillus nidulans results in a mitotic block in anaphase characterized by a defective mitosis. Mutation in bimD also confers, at temperatures permissive for the mitotic arrest phenotype, an increased sensitivity to DNA damaging agents, including methyl methanesulfonate and ultraviolet light. In order to better understand the relationship between DNA damage and mitotic progression, the authors cloned the bimD gene from Aspergillus. A cosmid containing the bimD gene was identified among pools of cosmids by cotransformation with the nutritional selective pyrG gene of a strain carrying the recessive, temperature-sensitive lethal bimD6 mutation. The bimD gene encodes a predicted polypeptide of 166,000 daltons in mass and contains amino acid sequence motifs similar to those found in some DNA-binding transcription factors. These sequences include a basic domain followed by a leucine zipper, which together are called a bZIP motif, and a carboxyl-terminal domain enriched in acidic amino acids. Overexpression of the wild-type bimD protein resulted in an arrest of the nuclear division cycle that was reversible and determined to be in either the G[sub 1] or S phase of the cell cycle. The data suggest that bimD may play an essential regulatory role relating to DNA metabolism which is required for a successful mitosis. 7l refs., 7 figs., 1 tab.

• OSTI ID: 5425279
• Journal Information: Genetics; (United States), Vol. 134:4; ISSN 0016-6731
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ASPERGILLUS
GENE MUTATIONS
CELL CYCLE
INHIBITION
MITOSIS
ULTRAVIOLET RADIATION
BIOLOGICAL EFFECTS
DNA-CLONING
CELL DIVISION
CLONING
DNA HYBRIDIZATION
ELECTROMAGNETIC RADIATION
EUMYCOTA
FUNGI
HYBRIDIZATION
MUTATIONS
PLANTS
RADIATIONS
560130* - Radiation Effects on Microorganisms