Increase in Phi X174 DNA radiation sensitivity due to electric fields
The object of this research was to establish whether or not orientation of DNA in electric fields would result in a significant increase in its sensitivity to damage by ionizing radiation. The application of an external electric field simultaneously with gamma irradiation to an aqueous suspension of Phi X 174 (in the RFI form) is shown to increase significantly the number of strand breaks. Tritiated DNA allowed the number of single-strand breaks to be estimated from changes in the scintillation of electrophoretic gel band associated with the fastest mobility moiety. At 400 V ( approx. 2400 V/cm) the corrected increase (corrected for phoresis of DNA on the stainless steel plates) in the G-value yield is 38%. The increase in damage with field strength appears to follow the increase in reduced dichroism. Dichroism results correspond at 400 V to approximately 10% of the maximum orientation. These results support the conjecture that this significant increase in DNA-radiation interaction with an electric field is due to field-induced conformation changes in the molecule. Keywords: Polyelectrolytes, Polynucleotides, Polypeptides, Birefringence, Dipole, and Moments.
- Research Organization:
- Armed Forces Radiobiology Research Inst., Bethesda, MD (USA)
- OSTI ID:
- 5425221
- Report Number(s):
- AD-A-167135/3/XAB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
BIOLOGICAL RADIATION EFFECTS
RADIOSENSITIVITY
ELECTRIC FIELDS
RADIOSENSITIVITY EFFECTS
ALIGNMENT
BIREFRINGENCE
COLLOIDS
DICHROISM
IRRADIATION
LIQUIDS
NUCLEOTIDES
PEPTIDES
POLYMERS
REDUCTION
STRAND BREAKS
TRACER TECHNIQUES
TRITIUM
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CHEMICAL REACTIONS
DISPERSIONS
FLUIDS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RADIOISOTOPES
REFRACTION
YEARS LIVING RADIOISOTOPES
560121* - Radiation Effects on Cells- External Source- (-1987)
560400 - Other Environmental Pollutant Effects