Staphylococcal protein A primed leukocytes enhance the autologous mixed lymphocyte reaction

Journal Article · Tue Apr 01 00:00:00 EST 1986 · Am. J. Med. Sci.; (United States)

DOI:https://doi.org/10.1097/00000441-198604000-00007· OSTI ID:5425127

Berk, G I; Lederman, M M; Liebman, M L; Ellner, J J

Human peripheral blood mononuclear cells (PBMC) were preincubated for 3 days in medium alone or with various mitogens then washed and irradiated. The preincubated cells then were cultured with autologous T-cells in an autologous mixed lymphocyte reaction (AMLR). Staphylococcal protein A (SPA) pretreatment of PBMC enhanced autologous T-lymphocyte proliferation from 1375 +/- 321 cpm (mean +/- SEM untreated PBMC) to 42,467 +/- 7985 cpm (SPA primed PBMC) (p less than 0.01). The ability of SPA treated PBMC to enhance the AMLR was not simply a reflection of their proliferation in preculture, as PBMC precultured with phytohemagglutinin and concanavalin A showed greater proliferation than SPA-treated PBMC yet only minimally enhanced the AMLR. Kinetic studies and pre-exposure of PBMC to graded doses of gamma radiation showed that SPA augmentation of the AMLR was mediated by 2 components which differed in kinetics and radiosensitivity. Although incubation of PBMC with SPA did not increase the percentage of cells with detectable surface Ia antigen, SPA did increase the density of Ia in the preincubated cells. Cell separation studies revealed that SPA enhancement of the AMLR was not mediated by T-cells, but was mediated by a non-adherent non-E-rosetting fraction of cells. SPA enhancement of the AMLR was associated with an increased Ia density in the stimulator population but not with an increase in Ia positive cells and was mediated by proliferation-dependent and proliferation-independent mechanisms.

• Research Organization: Case Western Reserve Univ. School of Medicine, Cleveland, OH
• OSTI ID: 5425127
• Journal Information: Am. J. Med. Sci.; (United States), Vol. 4
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LYMPHOCYTES
CELL PROLIFERATION
IMMUNE REACTIONS
PROTEINS
RADIOSENSITIVITY EFFECTS
ANTIGENS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CONCANAVALIN
GAMMA RADIATION
IRRADIATION
MITOGENS
PHYTOHEMAGGLUTININ
STAPHYLOCOCCUS
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
HEMAGGLUTININS
IONIZING RADIATIONS
LECTINS
LEUKOCYTES
MATERIALS
MICROORGANISMS
MUCOPROTEINS
ORGANIC COMPOUNDS
POLYSACCHARIDES
RADIATION EFFECTS
RADIATIONS
SACCHARIDES
SOMATIC CELLS
560121* - Radiation Effects on Cells- External Source- (-1987)