Characterization of the transport of. cap alpha. -methylaminoisobutyric acid by a human intestinal cell line (HT-29)

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5416523

Bergin, L; Dantzig, A H

Under certain growth conditions, the human colon adenocarcinoma cell line HT-29 exhibits intestinal enterocyte-like properties. The differentiated cells possess a brush border with the enzyme markers (aminopeptidase and sucrase) normally associated with the intestine. To aid in the characterization of the transport properties of these cells, the uptake of a non-metabolizable amino acid analog, /sup 14/C-..cap alpha..-methylaminoisobutyric acid (MeAIB) as examined in the HT-29-Al subclone which possesses a brush border. The cells exhibited a time-dependent uptake of MeAIB which was concentrative and sodium-dependent. The pH optimum for uptake was about 7.8. Uptake was inhibited by low temperature, 1 mM ouabain, or 0.5 mM dinitrophenol. A 1 hr-preincubation of the cells in an isotonic KCl solution resulted in a decreased uptake rate, suggesting that a negative membrane potential is important for MeAIB uptake. The rate of 0.5 mM MeABIB uptake was inhibited by 40 to 90% by 5 mM of certain small neutral amino acids such as Ala, Ser, Pro, Gly, met but not by acidic or basic amino acids such as Asp, Glu, Arg or Lys. The uptake of MeAIB appears to be mediated by an amino acid transport carrier similar to the A-system described previously for Chinese hamster ovary cells.

• Research Organization: Lilly Research Labs., Indianapolis, IN
• OSTI ID: 5416523
• Report Number(s): CONF-8604222-; TRN: 86-030513
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ISOBUTYRIC ACID
MEMBRANE TRANSPORT
AMINO ACIDS
CARBON 14 COMPOUNDS
CARCINOMAS
LARGE INTESTINE
MAN
PH VALUE
TEMPERATURE DEPENDENCE
TRACER TECHNIQUES
TUMOR CELLS
ANIMAL CELLS
ANIMALS
BODY
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
DISEASES
GASTROINTESTINAL TRACT
INTESTINES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
VERTEBRATES
550901* - Pathology- Tracer Techniques