Use of intact rat brain cells as a model to study regulation of muscarinic acetylcholine receptors
Intact rat brain cells were dissociated and used to study the regulation of muscarinic acetylcholine receptors upon exposure to muscarinic receptor agonists. Incubation of cells with carbamylcholine resulted in a time-dependent decrease in subsequent (/sup 3/H)N-methylscopolamine specific binding, an effect which reached a steady state after 3 hr at 37/sup 0/C. This effect of carbamylcholine was dependent on the concentration of the agonist in the incubation medium and was due to a reduction in the maximal binding capacity of the receptor with no decrease in the affinity of the remaining receptors. This preparation might be useful in future studies to elucidate the mechanisms underlying the regulation of muscarinic acetylcholine receptors in the central nervous system. 20 references, 3 tables.
- Research Organization:
- Univ. of Maryland School of Pharmacy, Baltimore
- OSTI ID:
- 5416383
- Journal Information:
- Life Sci.; (United States), Vol. 37:6
- Country of Publication:
- United States
- Language:
- English
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ACETYLCHOLINE
RECEPTORS
AFFINITY
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DOSE-RESPONSE RELATIONSHIPS
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STEADY-STATE CONDITIONS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
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DRUGS
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ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
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550201* - Biochemistry- Tracer Techniques