Effect of dietary fat intake on the kinetics of T cell cytotoxicity in C3H/OUJ mice
Seventy-two weanling female C3H/OUJ mice fed an AIN-76 diet containing either 12 or 40% of soybean oil (SBO) kcal for 12 wks showed no significant differences in body or spleen weight, caloric intake, or total spleen cell number. All mice were immunized with 3 x 10/sup 7/ P815 cells 10 days prior to splenectomy. Splenocytes (10/sup 6/) from 2 mice/treatment were cocultured for up to 10 hours with 12 concentrations of /sup 51/Cr-P815 cells. Killing was linear between 1 and 4 hours and initial velocity (V/sub 0/: P815 cells killed/hr) was determined from plots of cells killed versus time. V/sub max/, the maximum rate of killing, and K/sub 1/2/, the apparent affinity constant were determined from plots of V/sub 0/ versus target cell concentrations. There were no significant differences in the K/sub 1/2/ between treatments however V/sub max/ (x 10/sup -4/) was significantly lower (1.4 +/- 0.2 for 40% of kcal from SBO vs 2.3 +/- 0.4 for 12%). Assays of serum fatty acids and choline and ethanolamine phosphoglycerides in immune splenocytes showed a significant rise in linoleic acid and a decline in oleic acid in sera of mice fed 40% SBO kcal. T-cytotoxic cells (T/sub c/) from 40% SBO kcal mice had lower killing capacity (lower V/sub max/) without changes in apparent affinity for their targets (no difference in K/sub 1/2/). The decline in killing capacity appears unrelated to splenocyte membrane fatty acid changes and may be due to fewer T/sub c/ cells or their lower lytic efficiency with 40% SBO kcal.
- Research Organization:
- Univ. of Illinois College of Medicine, Urbana
- OSTI ID:
- 5416298
- Report Number(s):
- CONF-8604222-; TRN: 86-030539
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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551001* - Physiological Systems- Tracer Techniques