Pulse radiolysis studies of the interactions of the sulfhydryl compound dithiothreitol and sugars
Pulse radiolysis studies of the hydrogen atom transfer (repair) reaction from the sulfhydryl-containing (RSH) compound dithiothreitol (DTT) to the DNA sugar deoxyribose and to several related sugars have been undertaken. The H transfer reaction is measured by monitoring the transient absorbance of the radical-anion RSSR/sup -/. The H atom transfer reactions for some sugars were fitted by a single time exponential function, but other sugars exhibited both a fast and a slow component to the reaction. The maximum extent of total repair varied from 60% for ribose-5-phosphate to 100% for 2-deoxyglucose. The rate of repair, the extent of repair, and the appearance of more than one component of repair seem to depend on several factors. The biological relevance of the reactions studied herein is discussed and the rates obtained are compared with rates for repair of damage in certain radiobiological systems.
- Research Organization:
- Mayo Clinic, Rochester, MN
- OSTI ID:
- 5413040
- Journal Information:
- Radiat. Res.; (United States), Vol. 103:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DEOXYRIBOSE
HYDROGEN TRANSFER
RADIOLYSIS
DITHIOLS
CHEMICAL DOSEMETERS
DNA
ELECTRONS
PULSED IRRADIATION
REPAIR
SULFHYDRYL RADICALS
ALDEHYDES
CARBOHYDRATES
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
DOSEMETERS
ELEMENTARY PARTICLES
FERMIONS
IRRADIATION
LEPTONS
MEASURING INSTRUMENTS
MONOSACCHARIDES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PENTOSES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADICALS
REAGENTS
SACCHARIDES
THIOLS
400600* - Radiation Chemistry