Study of the function of sarcoplasmic reticulum of vascular smooth muscle during activation due to depolarization-induced calcium influx

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:5412681

Hwang, K S

The role of sarcoplasmic reticulum (SR) in vascular smooth muscle was evaluated with respect to regulation of myoplasmic Ca{sup 2+} during the Ca{sup 2+} entry induced by depolarization. Calcium agonist, Bay K8644, stimulated Ca{sup 2+} influx as well as tension in physiological salt solution, (PSS) in contrast to the priming effects due to the depolarization originally reported. Disparity, however, was found between the Ca{sup 2+} entered and tension developed. Correlation between the tension and {sup 45}Ca influx showed a typical threshold phenomenon; the basal Ca{sup 2+} influx can be raised to a certain level (25%) without tension induction, after which a minor increase in Ca{sup 2+} influx produced significant tension. This subthreshold Ca{sup 2+} influx was found accumulated in the caffeine-sensitive Ca stores, the SR. This confirmed the dependency of tension on the rate of Ca{sup 2+} entry demonstrated by a previous report.

• Research Organization: Miami Univ., Coral Gables, FL (USA)
• OSTI ID: 5412681
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CALCIUM COMPOUNDS
MEMBRANE TRANSPORT
SARCOPLASMIC RETICULUM
BIOLOGICAL FUNCTIONS
CALCIUM 45
DEPOLARIZATION
MUSCLES
TRACER TECHNIQUES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM ISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
ENDOPLASMIC RETICULUM
EVEN-ODD NUCLEI
FUNCTIONS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
ORGANOIDS
RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques