Comparative effect of recombinant IL-1, -2, -3, -4, and -6, IFN-gamma, granulocyte-macrophage-colony-stimulating factor, tumor necrosis factor-alpha, and histamine-releasing factors on the secretion of histamine from basophils

Journal Article · Mon May 15 00:00:00 EDT 1989 · Journal of Immunology; (USA)

OSTI ID:5412491

Alam, R; Welter, J B; Forsythe, P A; Lett-Brown, M A; Grant, J A ^[1]

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1. Univ. of Texas Medical Branch, Galveston (USA)

Most cytokines possess multiple biologic activities. This study was undertaken to investigate the effect of rIL-1 beta, -2, -3, -4 and -6, IFN-gamma, TNF-alpha, and granulocyte-macrophage (GM)-CSF on basophils from 16 donors and the amount of histamine released was compared with that by partially purified mononuclear cell-derived histamine-releasing factor (HRF) and anti-IgE. We found that only IL-3 and GM-CSF at relatively high doses (50 to 500 ng/ml) released small amounts of histamine (3 to 14%) from two allergic donors. In contrast, both HRF and anti-IgE released significant amounts of histamine from all donors. Other cytokines did not release any measurable quantity of histamine. Simultaneous addition of several cytokines to the basophils also failed to release histamine. IL-3, GM-CSF, and IL-1 can also release histamine at lower concentrations (less than 5 ng/ml) when incubated with basophils in the presence of D{sub 2}O. Basophils from 6 out of 13 allergic donors released histamine in response to IL-3, whereas three donors responded to IL-1 beta and two responded to GM-CSF. The results of this study demonstrated that although IL-3 and GM-CSF release small amounts of histamine only from a select group of allergic patients, mononuclear cell-derived HRF is more potent in their action and release histamine from normals as well as allergic patients.

• OSTI ID: 5412491
• Journal Information: Journal of Immunology; (USA), Vol. 142:10; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
BIOCHEMICAL REACTION KINETICS
HISTAMINE
SECRETION
IMMUNE SYSTEM DISEASES
PATHOGENESIS
ALLERGY
BASOPHILS
COLONY FORMATION
COMPARATIVE EVALUATIONS
DEUTERIUM
INTERFERON
LYMPHOKINES
MICE
MONOCLONAL ANTIBODIES
PATIENTS
PROTEINS
TRACER TECHNIQUES
AMINES
ANIMALS
ANTIBODIES
AZOLES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DISEASES
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
IMIDAZOLES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
LIGHT NUCLEI
MAMMALS
MATERIALS
MITOGENS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGICAL CHANGES
REACTION KINETICS
RODENTS
STABLE ISOTOPES
VERTEBRATES
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