(/sup 3/H)-(Thr4,Gly7)OT: a highly selective ligand for central and peripheral OT receptors
Oxytocin receptors in rat hippocampal synaptic plasma membranes were compared with mammary gland and uterine oxytocin receptors. For this purpose, a highly specific oxytocic agonist (Thr4,Gly7)oxytocin was tritiated. We demonstrated that this ligand labels oxytocin receptors selectively. Scatchard analyses revealed a high affinity for all the oxytocin receptors investigated, with equilibrium dissociation constants between 1.0 and 2.0 nM. Binding appeared to take place at a single population of receptor sites. Competition experiments confirmed the high affinity of arginine vasopressin for hippocampal oxytocin receptors but also revealed that mammary gland and uterine oxytocin receptors do not discriminate more efficiently between oxytocin and arginine vasopressin. This lack in specificity is not affected by applying different concentrations of Mg ions.
- Research Organization:
- Centre National de la Recherche Scientifique-Institut National de la Sante et de la Recherche Medicale de Pharmacologie-Endocrinologie, Montpellier, France
- OSTI ID:
- 5412212
- Journal Information:
- Am. J. Physiol.; (United States), Vol. 254
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
OXYTOCIN
BIOCHEMICAL REACTION KINETICS
RECEPTORS
AFFINITY
BLOOD PLASMA
CELL MEMBRANES
COMPARATIVE EVALUATIONS
HIPPOCAMPUS
LIGANDS
MAMMARY GLANDS
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UTERUS
VASOPRESSIN
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
BRAIN
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
FEMALE GENITALS
GLANDS
HORMONES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques