Evidence that cellulolysis by an anaerobic ruminal fungus is catabolite regulated by glucose, cellobiose, and soluble starch

Journal Article · Mon Oct 01 00:00:00 EDT 1990 · Applied and Environmental Microbiology; (United States)

OSTI ID:5409934

Morrison, M; Mackie, R I; Kistner, A ^[1]

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1. Animal and Dairy Science Research Institute, Irene (South Africa)

A Piromyces-like ruminal fungus was used to study preferential carbohydrate utilization of (U-{sup 14}C)cellulose, both alone and in combination with several soluble sugars. For cells grown on cellulose alone, cellulolytic activity was immediate and, initially, greater than that observed in the presence of added carbohydrate. Cellulolytic activity remained minimal in cultures containing cellulose plus glucose or cellobiose until the soluble sugar was depleted. Soluble starch also regulated cellulose activity but to a lesser extent. The results presented suggest that some fungal cellulases are susceptible to catabolite regulatory mechanisms.

• OSTI ID: 5409934
• Journal Information: Applied and Environmental Microbiology; (United States), Vol. 56:10; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
60 APPLIED LIFE SCIENCES
CELLULASE
BIOCHEMICAL REACTION KINETICS
FUNGI
CELLULOLYTIC ACTIVITY
CARBON 14 COMPOUNDS
CATABOLISM
CELLOBIOSE
CELLULOSE
ENZYME ACTIVITY
GLUCOSE
RUMINANTS
STARCH
STOMACH
TRACER TECHNIQUES
ALDEHYDES
ANIMALS
BODY
CARBOHYDRATES
CARBON COMPOUNDS
DIGESTIVE SYSTEM
DISACCHARIDES
ENZYMES
GASTROINTESTINAL TRACT
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PLANTS
POLYSACCHARIDES
REACTION KINETICS
REAGENTS
SACCHARIDES
VERTEBRATES
550501* - Metabolism- Tracer Techniques
090900 - Biomass Fuels- Processing- (1990-)
553005 - Agriculture & Food Technology- Animal Husbandry- (1987-)