Nucleotide sequencing and characterization of the genes encoding benzene oxidation enzymes of Pseudomonas putida

Journal Article · Sun Nov 01 00:00:00 EST 1987 · J. Bacteriol.; (United States)

OSTI ID:5409226

Irie, S; Doi, S; Yorifuji, T; Takagi, M; Yano, K

The nucleotide sequence of the genes from Pseudomonas putida encoding oxidation of benzene to catechol was determined. Five open reading frames were found in the sequence. Four corresponding protein molecules were detected by a DNA-directed in vitro translation system. Escherichia coli cells containing the fragment with the four open reading frames transformed benzene to cis-benzene glycol, which is an intermediate of the oxidation of benzene to catechol. The relation between the product of each cistron and the components of the benzene oxidation enzyme system is discussed.

• Research Organization: Univ. of Tokyo, Japan
• OSTI ID: 5409226
• Journal Information: J. Bacteriol.; (United States), Vol. 169:11
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BENZENE
BIODEGRADATION
ENZYMES
DNA SEQUENCING
GENETIC MAPPING
PSEUDOMONAS
MOLECULAR BIOLOGY
ENZYME ACTIVITY
GENES
PYROCATECHOL
AROMATICS
BACTERIA
CHEMICAL REACTIONS
DECOMPOSITION
DEVELOPERS
HYDROCARBONS
HYDROXY COMPOUNDS
MAPPING
MICROORGANISMS
ORGANIC COMPOUNDS
PHENOLS
POLYPHENOLS
STRUCTURAL CHEMICAL ANALYSIS
550500* - Metabolism
550200 - Biochemistry
550400 - Genetics
550700 - Microbiology