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Title: Red blood cell calcium homeostasis in patients with end-stage renal disease

Abstract

Low cell calcium level is essential for preservation of red blood cell (RBC) membrane deformability and survival. RBCs from patients with end-stage renal disease (ESRD) demonstrate reduction in membrane deformability, possibly as a result of increased RBC cellular calcium level. To evaluate calcium homeostasis in RBCs from patients with ESRD, we measured cell calcium level, basal and calmodulin-stimulated calcium-stimulated Mg-dependent ATPase (CaATPase) activity, and calcium 45 efflux were measured before and after hemodialysis. The in vitro effect of uremic plasma and of urea on CaATPase activity of normal RBCs was tested, and 45Ca influx into RBCs of patients undergoing hemodialysis also was determined. A morphologic evaluation of red cells from patients with ESRD was performed with a scanning electron microscope. RBC calcium level in patients (mean +/- SEM 21.2 +/- 2.8 mumol/L of cells; n = 28) was higher than in controls (4.9 +/- 0.3 mumol/L of cells; n = 24; p less than 0.001). Hemodialysis had no effect on cell calcium level. Both basal and calmodulin-stimulated RBC CaATPase activities in patients with ESRD (n = 9) were reduced by approximately 50% (p less than 0.01), but after hemodialysis, enzyme activity returned to normal. 45Ca efflux from calcium-loaded cells, whichmore » was 2574.0 +/- 217.0 mumol/L of cells per 0.5 hours before hemodialysis, increased to 3140.7 +/- 206.8 mumol/L of cells per 0.5 hours after hemodialysis (p less than 0.005). In vitro incubation of normal RBCs with uremic plasma depressed CaATPase activity, but incubation with urea had no effect. RBCs of patients with ESRD revealed increased 45Ca influx, 7.63 +/- 1.15 mumol/L of cells per hour versus 4.61 +/- 0.39 mumol/L of cells per hour (p less than 0.025). RBCs of patients revealed a high incidence of spherocytosis and echynocytosis, which correlated with a high cell calcium level (r = 0.894, p less than 0.01).« less

Authors:
; ; ; ;  [1]
  1. Hasharon Hospital, Petah-Tiqva (Israel)
Publication Date:
OSTI Identifier:
5408335
Resource Type:
Journal Article
Journal Name:
Journal of Laboratory and Clinical Medicine; (USA)
Additional Journal Information:
Journal Volume: 114:3; Journal ID: ISSN 0022-2143
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ATP-ASE; ENZYME ACTIVITY; CALCIUM; HOMEOSTASIS; UROGENITAL SYSTEM DISEASES; PATHOGENESIS; CALCIUM 45; CELL MEMBRANES; DIALYSIS; ERYTHROCYTES; KIDNEYS; MEMBRANE TRANSPORT; PATIENTS; SCANNING ELECTRON MICROSCOPY; TRACER TECHNIQUES; ACID ANHYDRASES; ALKALINE EARTH ISOTOPES; ALKALINE EARTH METALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CALCIUM ISOTOPES; CELL CONSTITUENTS; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON MICROSCOPY; ELEMENTS; ENZYMES; EVEN-ODD NUCLEI; HYDROLASES; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; MATERIALS; MEMBRANES; METALS; MICROSCOPY; NUCLEI; ORGANS; PHOSPHOHYDROLASES; RADIOISOTOPES; SEPARATION PROCESSES; 550901* - Pathology- Tracer Techniques

Citation Formats

Gafter, U, Malachi, T, Barak, H, Djaldetti, M, and Levi, J. Red blood cell calcium homeostasis in patients with end-stage renal disease. United States: N. p., 1989. Web.
Gafter, U, Malachi, T, Barak, H, Djaldetti, M, & Levi, J. Red blood cell calcium homeostasis in patients with end-stage renal disease. United States.
Gafter, U, Malachi, T, Barak, H, Djaldetti, M, and Levi, J. 1989. "Red blood cell calcium homeostasis in patients with end-stage renal disease". United States.
@article{osti_5408335,
title = {Red blood cell calcium homeostasis in patients with end-stage renal disease},
author = {Gafter, U and Malachi, T and Barak, H and Djaldetti, M and Levi, J},
abstractNote = {Low cell calcium level is essential for preservation of red blood cell (RBC) membrane deformability and survival. RBCs from patients with end-stage renal disease (ESRD) demonstrate reduction in membrane deformability, possibly as a result of increased RBC cellular calcium level. To evaluate calcium homeostasis in RBCs from patients with ESRD, we measured cell calcium level, basal and calmodulin-stimulated calcium-stimulated Mg-dependent ATPase (CaATPase) activity, and calcium 45 efflux were measured before and after hemodialysis. The in vitro effect of uremic plasma and of urea on CaATPase activity of normal RBCs was tested, and 45Ca influx into RBCs of patients undergoing hemodialysis also was determined. A morphologic evaluation of red cells from patients with ESRD was performed with a scanning electron microscope. RBC calcium level in patients (mean +/- SEM 21.2 +/- 2.8 mumol/L of cells; n = 28) was higher than in controls (4.9 +/- 0.3 mumol/L of cells; n = 24; p less than 0.001). Hemodialysis had no effect on cell calcium level. Both basal and calmodulin-stimulated RBC CaATPase activities in patients with ESRD (n = 9) were reduced by approximately 50% (p less than 0.01), but after hemodialysis, enzyme activity returned to normal. 45Ca efflux from calcium-loaded cells, which was 2574.0 +/- 217.0 mumol/L of cells per 0.5 hours before hemodialysis, increased to 3140.7 +/- 206.8 mumol/L of cells per 0.5 hours after hemodialysis (p less than 0.005). In vitro incubation of normal RBCs with uremic plasma depressed CaATPase activity, but incubation with urea had no effect. RBCs of patients with ESRD revealed increased 45Ca influx, 7.63 +/- 1.15 mumol/L of cells per hour versus 4.61 +/- 0.39 mumol/L of cells per hour (p less than 0.025). RBCs of patients revealed a high incidence of spherocytosis and echynocytosis, which correlated with a high cell calcium level (r = 0.894, p less than 0.01).},
doi = {},
url = {https://www.osti.gov/biblio/5408335}, journal = {Journal of Laboratory and Clinical Medicine; (USA)},
issn = {0022-2143},
number = ,
volume = 114:3,
place = {United States},
year = {Fri Sep 01 00:00:00 EDT 1989},
month = {Fri Sep 01 00:00:00 EDT 1989}
}