Cloning of the gene encoding the. delta. subunit of the human T-cell receptor reveals its physical organization within the. alpha. -subunit locus and its involvement in chromosome translocations in T-cell malignancy

Journal Article · Wed Jun 01 00:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.11.3933· OSTI ID:5404734

Isobe, M; Russo, G; Haluska, F G; Croce, C M ^[1]

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1. Wistar Institute of Anatomy and Biology, Philadelphia, PA (USA)

By taking advantage of chromosomal walking techniques, the authors have obtained clones that encompass the T-cell receptor (TCR) {delta}-chain gene. They analyzed clones spanning the entire J{sub {alpha}} region extending 115 kilobases 5{prime} of the TCR {alpha}-chain constant region and have shown that the TCR {delta}-chain gene is located over 80 kilobases 5{prime} of C{sub {alpha}}. TCR {delta}-chain gene is rearranged in the {gamma}/{delta}-expressing T-cell line Peer and is deleted in {alpha}/{beta}-expressing T-cell lines. Sequence analysis of portions of this genomic region demonstrates its identity with previously described cDNA clones corresponding to the C{sub {delta}} and J{sub {delta}} segments. Furthermore, they have analyzed a t(8;14)-(q24;q11) chromosome translocation from a T-cell leukemia and have shown that the J{sub {delta}} segment is rearranged in cells deriving from this tumor and probably directly involved in the translocation. Thus, the newly clones TCR {delta} chain is implicated in the genesis of chromosome translocations in T-cell malignancies carrying cytogenetic abnormalities of band 14q11.

• OSTI ID: 5404734
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:11; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
DNA-CLONING
LEUKEMOGENESIS
MOLECULAR BIOLOGY
RECEPTORS
GENETIC MAPPING
CHROMOSOMAL ABERRATIONS
DNA SEQUENCING
LEUKEMIA
MOLECULAR STRUCTURE
OLIGONUCLEOTIDES
TUMOR CELLS
ANIMAL CELLS
CARCINOGENESIS
CLONING
DISEASES
DNA HYBRIDIZATION
HEMIC DISEASES
HYBRIDIZATION
IMMUNE SYSTEM DISEASES
MAPPING
MEMBRANE PROTEINS
MUTATIONS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATHOGENESIS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
550401* - Genetics- Tracer Techniques