Porphyrin-laser photodynamic induction of focal brain necrosis

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Journal of Neuropathology and Experimental Neurology; (USA)

DOI:https://doi.org/10.1097/00005072-198909000-00005· OSTI ID:5403832

Stroop, W G; Battles, E J; Townsend, J J; Schaefer, D C; Baringer, J R; Straight, R C ^[1]

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1. VA Medical Center, Salt Lake City, UT (USA)

A noninvasive photodynamic method has been developed to produce focal brain necrosis using porphyrin activated in vivo with laser light. After peripheral injection of the photosensitive porphyrin derivative, Photofrin I, mice were irradiated on the posterior lateral aspect of the head through the intact depilated scalp with 632 nm argon-dye laser light. Animals were studied at one, two and seven days after irradiation. Blood-brain barrier damage was detected by the intravenous injection of Evans blue, horseradish peroxidase and heterologous immunoglobulins. At one and two days after irradiation, the lesions were characterized by extravasation of immunoglobulin and Evans blue, and by edema, ischemia and infiltration by monocytes. On the seventh day after irradiation, the lesion was smaller than it had been two days after irradiation, and had reactive changes at its edges and coagulative necrosis at its center. Extravasation of Evans blue and immunoglobulin was markedly reduced by the seventh day after irradiation, but uptake of horseradish peroxidase by macrophages located at the periphery of the lesion was evident.

• OSTI ID: 5403832
• Journal Information: Journal of Neuropathology and Experimental Neurology; (USA), Vol. 48:5; ISSN 0022-3069
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BRAIN
NECROSIS
LASER RADIATION
BIOLOGICAL EFFECTS
LASERS
MICE
PEROXIDASES
PORPHYRINS
ANIMALS
BODY
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HETEROCYCLIC COMPOUNDS
MAMMALS
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ORGANS
OXIDOREDUCTASES
PATHOLOGICAL CHANGES
RADIATIONS
RODENTS
VERTEBRATES
560400* - Other Environmental Pollutant Effects