Partitioning of copper among copper-binding proteins in the mussel Mytilus edulis exposed to soluble copper
Partitioning of copper among copper-binding proteins was evaluated in digestive glands of Mytilus edulis exposed to soluble copper. Groups of mussels were held in flow-through bioassay systems and exposed to either approx. 1 (control) or 25 ..mu..g Cu/L for as long as 21 weeks. At 3-week intervals, groups of 25 mussels were removed and the digestive glands were analyzed for copper-binding proteins by gel-permeation chromatography and atomic absorption spectrometry. Chronic exposure to copper resulted in increased amounts of copper in the low molecular-weight (LMW) protein fraction, which contains metallothionein-like proteins, and in the high molecular-weight (HMW) protein fraction, which contains metalloenzymes. Concentrations of copper in the LMW protein fraction increased and then appeared to plateau with long exposure times, whereas those in the HMW protein fraction continued to increase with exposure time.
- Research Organization:
- Lawrence Livermore National Lab., CA (USA)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 5402774
- Report Number(s):
- UCRL-88687; CONF-8310230-1; ON: DE84004361
- Resource Relation:
- Conference: Environmental health aspects of estuarine research symposium, Virginia Beach, VA, USA, 22 Oct 1983
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
CADMIUM
QUANTITATIVE CHEMICAL ANALYSIS
COPPER
ENZYMES
LIQUID COLUMN CHROMATOGRAPHY
METALLOTHIONEIN
ZINC
ABSORPTION SPECTROSCOPY
EXPERIMENTAL DATA
GASTROINTESTINAL TRACT
MOLLUSCS
ANIMALS
AQUATIC ORGANISMS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
DIGESTIVE SYSTEM
ELEMENTS
INFORMATION
INVERTEBRATES
METALLOPROTEINS
METALS
NUMERICAL DATA
ORGANIC COMPOUNDS
PROTEINS
SEPARATION PROCESSES
SPECTROSCOPY
TRANSITION ELEMENTS
560304* - Chemicals Metabolism & Toxicology- Invertebrates- (-1987)
550500 - Metabolism