Radioisotope dilution technique for determination of vitamin B/sub 12/ in foods

Journal Article · Fri Jan 01 00:00:00 EST 1982 · J. Assoc. Off. Anal. Chem.; (United States)

OSTI ID:5402470

Casey, P J; Speckman, K R; Ebert, F J; Hobbs, W E

A radioisotope dilution (RID) method for the determination of vitamin B/sub 12/ is presented. The method combines a standard extraction procedure (AOAC 43.108, 12th ed.) with a commercially available RID assay kit. The method was evaluated on a wide range of fortified and unfortified food products. Recovery studies on both groups yielded average recoveries of 98.1 and 95.8%, respectively. Reproducibility data generated from replicate analysis on both groups gave a relative standard deviation of 6.9% for the fortified group and 9.2% for the unfortified group. For the samples studied, the mean vitamin B/sub 12/ content determined by the RID method was 8.01 ..mu../100 g vs a mean of 7.54 ..mu..g/100 g by the AOAC microbiological method; the correlation coefficient was r = 0.983.

• Research Organization: General Mills, Minneapolis, MN
• OSTI ID: 5402470
• Journal Information: J. Assoc. Off. Anal. Chem.; (United States), Vol. 65:1
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
FOOD
QUALITATIVE CHEMICAL ANALYSIS
VITAMIN B-12
ISOTOPE DILUTION
CHEMICAL ANALYSIS
EXTRACTION
LABELLED COMPOUNDS
DRUGS
HEMATINICS
HEMATOLOGIC AGENTS
ISOTOPE APPLICATIONS
SEPARATION PROCESSES
TRACER TECHNIQUES
VITAMIN B GROUP
VITAMINS
553001* - Agriculture & Food Technology- Tracer Techniques- (-1987)
400103 - Radiometric & Radiochemical Procedures- (-1987)