Effects of cadmium on Drosophila: toxicity, proteins, and transfer RNAs
- Oak Ridge National Lab., TN
An animal model with well-defined genetic and biochemical characteristics is needed for a detailed understanding of the mechanism of toxicity by metal ions. Drosophila melanogaster was used in the present study to demonstrate a number of responses to Cd/sup 2 +/, including lethality, age-related changes in resistance, alterations of the normal developmental changes in proteins, and alterations in specific transfer RNAs. Genotype-specific differences in resistance to Cd/sup 2 +/ were found: the v; bw strain was 5-10 times more resistant than su(s)/sup 2/v; bw for developmental exposure; upon treatment of the young adults the differences were in the same direction, but the sensitivities differed by only two- to three-fold. The adult fly became more sensitive to Cd/sup 2 +/ as it aged through 2 weeks, but changed little thereafter.The electrophoretic patterns of proteins of adult flies underwent changes during aging from 1 to 8 days; these changes were markedly altered by 0.55 mM CdCl/sub 2/ but not by 0.74 mM ZnCl/sub 2/ in the medium on which the flies were maintained. The appearance of queuosine-containing tRNA was stimulated by CdCl/sub 2/ (0.05-0.8 mM) in the growth medium, but not by ZnCl/sub 2/ (0.07-1.1 mM).Further studies involving D. melanogaster should be useful in defining specific interactions of toxic metal ions with macromolecules to enhance the understanding of the toxic effects of these and similar pollutants.
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5400436
- Journal Information:
- Toxicol. Appl. Pharmacol.; (United States), Vol. 60
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CADMIUM CHLORIDES
BIOLOGICAL EFFECTS
TOXICITY
GENOTYPE
SENSITIVITY
TRANSFER RNA
STIMULATION
CATIONS
DOSE-RESPONSE RELATIONSHIPS
DROSOPHILA
ELECTROPHORESIS
LETHAL DOSES
MORTALITY
PROTEINS
ZINC CHLORIDES
ANIMALS
ARTHROPODS
CADMIUM COMPOUNDS
CADMIUM HALIDES
CHARGED PARTICLES
CHLORIDES
CHLORINE COMPOUNDS
DIPTERA
DOSES
FLIES
FRUIT FLIES
HALIDES
HALOGEN COMPOUNDS
INSECTS
INVERTEBRATES
IONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RNA
ZINC COMPOUNDS
ZINC HALIDES
560304* - Chemicals Metabolism & Toxicology- Invertebrates- (-1987)