Effect of nitrogen nutrition on endosperm protein synthesis in wild and cultivated barley grown in spike culture
- Weizmann Institute of Science, Rehovot (Israel)
In normal growth conditions, total protein percent, in the endosperm at maturity in barley cultivar Hordeum vulgare L. cv Ruth was about 14%, whereas in an accession of wild barley, Hordeum spontaneum Koch line 297, it was about 28%. Spike culture experiments were conducted to ascertain whether there were basic differences between the two genotypes under conditions of widely different nitrogen supply. Spikes of each genotype were grown from 8 to 25 days after flowering in in vitro culture in a growth medium containing 0 to 4 grams per liter nitrogen supplied as NH{sub 4}NO{sub 3}. Spikes were pulse-labeled at intervals from 12 to 24 days after flowering with 3.7 megabecquerel of ({sup 3}H)leucine to determine relative rates of synthesis of hordein-1 and hordein-2 polypedtides. At low nitrogen levels Ruth had a lower protein content than 297, but at increasing nitrogen levels its protein content increased rapidly and reached a maximum (35%) higher than 297 (30%). The relative contribution of the hordein fraction to total protein increased mainly with time, and hordein-1 to total hordein increased mainly with nitrogen level, in both genotypes. There appeared to be no fundamental limitations in the capacity of Ruth to accumulate protein: 297 appears to have a greater basal level of nitrogen availability under normal conditions.
- OSTI ID:
- 5400105
- Journal Information:
- Plant Physiology; (USA), Vol. 87:2; ISSN 0032-0889
- Country of Publication:
- United States
- Language:
- English
Similar Records
Division S-4 - soil fertility and plant nutrition: interactions of micronutrients (Mn, Fe, Mo, Cu, Zn) in barley grown on zinc-polluted soils
Effects of sulfur nutritional level on cadmium toxicity in barley
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BARLEY
PLANT GROWTH
NITRATES
BIOLOGICAL EFFECTS
PROTEINS
BIOSYNTHESIS
GENETIC VARIABILITY
GENOTYPE
LEUCINE
NUTRIENTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINO ACIDS
BIOLOGICAL VARIABILITY
CARBOXYLIC ACIDS
CEREALS
GRAMINEAE
GROWTH
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LILIOPSIDA
MAGNOLIOPHYTA
NITROGEN COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PLANTS
SYNTHESIS
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques