Gene structure and chromosomal localization of plasma kallikrein
- Clinical Research Institute of Montreal, Quebec (Canada)
- Inst. Pasteur, Paris (France)
- Groupe hospitalier de a Timone, Marseille (France)
Plasma kallikrein (Fletcher factor) is a hepatic serine proteinase that participates in the early phase of blood coagulation. From two genomic libraries, the authors succeeded to isolate four overlapping clones representing the entire rat plasma kallikrein gene. Using selective DNA sequencing, polymerase chain reactions, and restriction mapping, the authors demonstrated that the gene for rat plasma kallikrein was 22 kb in length. Similar to human factor XI the authors also found that the plasma kallikrein gene is composed of 15 exons and 14 introns. A potential transcription initiation step was determined by a novel application of the polymerase chain reaction technique. Computer analysis of the 5{prime}-promoter region of this gene revealed some putative control elements that might regulate the rat plasma kallikrein gene expression. These data and the results of chromosomal localization reported in the present study for mouse (chromosome 8) and human (chromosome 4) plasma kallikrein genes strongly corroborate a genic duplication event from a common ancestor to both plasma kallikrein and factor XI.
- OSTI ID:
- 5398887
- Journal Information:
- Biochemistry; (United States), Vol. 30:6; ISSN 0006-2960
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHROMOSOMES
AUTORADIOGRAPHY
OLIGONUCLEOTIDES
MOLECULAR STRUCTURE
SERINE PROTEINASES
GENETIC MAPPING
BLOOD COAGULATION
DNA HYBRIDIZATION
HETEROCHROMOSOMES
LIVER
BODY
DIGESTIVE SYSTEM
ENZYMES
GLANDS
HYBRIDIZATION
HYDROLASES
MAPPING
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
550201* - Biochemistry- Tracer Techniques