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Title: Possible genetic defects in regulation of glycosaminoglycans in patients with diabetic nephropathy

Abstract

The hypothesis of genetic defects in glycosaminoglycan (GAG) regulation among patients with insulin-dependent diabetes mellitus (IDDM) and nephropathy was assessed by studies in tissue cultures of fibroblasts obtained from 7 patients with normal urinary albumin excretion, 11 patients with diabetic nephropathy, and 6 nondiabetic control subjects. The incorporation of (2H) glucosamine and (35S) sulfate into hyaluronic acid (HA), chondroitin sulfate and dermatan sulfate (CS + DS), and heparan sulfate (HS) was measured in cells, matrix, and medium and related to micrograms of tissue protein. Large interindividual variations were seen in all three groups, and the incorporation of (3H) glucosamine into HA, CS + DS, and HS and (35S) sulfate into CS + DS and HS were not significantly different between the three groups. However, the fractional incorporation of (3H)glucosamine into HS was significantly reduced in diabetic patients with nephropathy compared with control subjects. This was the case not only when related to the total amount of GAGs (P = 0.014) but also when related to HA (P = 0.014). No significant difference was seen between control subjects and normoalbuminuric diabetic patients. The degree of N-sulfation of HS was not significantly different between the experimental groups. The results suggest that patientsmore » with diabetic nephropathy may suffer from deficiencies of coordinate regulation in the biosynthesis of GAG in fibroblasts, which may lead to a reduced density of HS in the extracellular matrix. If these changes reflect alterations in the biosynthesis of GAG from endothelial, myomedial, and mesangial cells, this observation may be relevant for the pathogenesis of severe diabetic complications.« less

Authors:
; ; ; ; ; ;  [1]
  1. Steno Memorial Hospital, Gentofte (Denmark)
Publication Date:
OSTI Identifier:
5396769
Resource Type:
Journal Article
Journal Name:
Diabetes; (United States)
Additional Journal Information:
Journal Volume: 40:6; Journal ID: ISSN 0012-1797
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; DIABETES MELLITUS; PATHOGENESIS; GLUCOSAMINE; BIOSYNTHESIS; DEUTERIUM; FIBROBLASTS; PATIENTS; SKIN; SULFATES; SULFUR 35; TRACER TECHNIQUES; TRITIUM COMPOUNDS; AMINES; ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CARBOHYDRATES; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DISEASES; ENDOCRINE DISEASES; EVEN-ODD NUCLEI; HEXOSAMINES; HEXOSES; HYDROGEN COMPOUNDS; HYDROGEN ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; METABOLIC DISEASES; MONOSACCHARIDES; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; OXYGEN COMPOUNDS; RADIOISOTOPES; SACCHARIDES; SOMATIC CELLS; STABLE ISOTOPES; SULFUR COMPOUNDS; SULFUR ISOTOPES; SYNTHESIS; 550901* - Pathology- Tracer Techniques

Citation Formats

Deckert, T, Horowitz, I M, Kofoed-Enevoldsen, A, Kjellen, L, Deckert, M, Lykkelund, C, and Burcharth, F. Possible genetic defects in regulation of glycosaminoglycans in patients with diabetic nephropathy. United States: N. p., 1991. Web. doi:10.2337/diab.40.6.764.
Deckert, T, Horowitz, I M, Kofoed-Enevoldsen, A, Kjellen, L, Deckert, M, Lykkelund, C, & Burcharth, F. Possible genetic defects in regulation of glycosaminoglycans in patients with diabetic nephropathy. United States. https://doi.org/10.2337/diab.40.6.764
Deckert, T, Horowitz, I M, Kofoed-Enevoldsen, A, Kjellen, L, Deckert, M, Lykkelund, C, and Burcharth, F. 1991. "Possible genetic defects in regulation of glycosaminoglycans in patients with diabetic nephropathy". United States. https://doi.org/10.2337/diab.40.6.764.
@article{osti_5396769,
title = {Possible genetic defects in regulation of glycosaminoglycans in patients with diabetic nephropathy},
author = {Deckert, T and Horowitz, I M and Kofoed-Enevoldsen, A and Kjellen, L and Deckert, M and Lykkelund, C and Burcharth, F},
abstractNote = {The hypothesis of genetic defects in glycosaminoglycan (GAG) regulation among patients with insulin-dependent diabetes mellitus (IDDM) and nephropathy was assessed by studies in tissue cultures of fibroblasts obtained from 7 patients with normal urinary albumin excretion, 11 patients with diabetic nephropathy, and 6 nondiabetic control subjects. The incorporation of (2H) glucosamine and (35S) sulfate into hyaluronic acid (HA), chondroitin sulfate and dermatan sulfate (CS + DS), and heparan sulfate (HS) was measured in cells, matrix, and medium and related to micrograms of tissue protein. Large interindividual variations were seen in all three groups, and the incorporation of (3H) glucosamine into HA, CS + DS, and HS and (35S) sulfate into CS + DS and HS were not significantly different between the three groups. However, the fractional incorporation of (3H)glucosamine into HS was significantly reduced in diabetic patients with nephropathy compared with control subjects. This was the case not only when related to the total amount of GAGs (P = 0.014) but also when related to HA (P = 0.014). No significant difference was seen between control subjects and normoalbuminuric diabetic patients. The degree of N-sulfation of HS was not significantly different between the experimental groups. The results suggest that patients with diabetic nephropathy may suffer from deficiencies of coordinate regulation in the biosynthesis of GAG in fibroblasts, which may lead to a reduced density of HS in the extracellular matrix. If these changes reflect alterations in the biosynthesis of GAG from endothelial, myomedial, and mesangial cells, this observation may be relevant for the pathogenesis of severe diabetic complications.},
doi = {10.2337/diab.40.6.764},
url = {https://www.osti.gov/biblio/5396769}, journal = {Diabetes; (United States)},
issn = {0012-1797},
number = ,
volume = 40:6,
place = {United States},
year = {Sat Jun 01 00:00:00 EDT 1991},
month = {Sat Jun 01 00:00:00 EDT 1991}
}