In vivo acylation of proteolipid protein and DM-20 in myelin and myelin subfractions of developing rat brain: immunoblot identification of acylated PLP and DM-20

Journal Article · Sun May 01 00:00:00 EDT 1983 · Neurochem. Res.; (United States)

DOI:https://doi.org/10.1007/BF00964704· OSTI ID:5394955

Garwood, M M; Gilbert, W R; Agrawal, H C

The acylation of proteolipid protein (PLP) was examined in myelin and myelin subfractions from rat brain during the active period of myelination. Proteolipid protein and DM-20 in myelin and myelin subfractions were readily acylated in developing rat brain 22 hours after intracerebral injection of (/sup 3/H)palmitic acid. No differences in the relative specific activity of PLP in myelin from 9-, 15-, and 30-day-old rat brains was observed; however, the relative specific activity of PLP in the heavy myelin subfraction tended to be higher than that in the light myelin subfraction. The acylation of PLP was confirmed by fluorography of immuno-stained cellulose nitrate sheets, clearly establishing that the acylated protein is in fact the oligodendroglial cell- and myelin-specific protein, PLP. Since PLP is acylated in the 9-day-old animal, when little compact myelin is present, it is possible that the acylation of PLP is a prerequisite for the incorporation of this protein into the myelin membrane.

• Research Organization: Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri
• OSTI ID: 5394955
• Journal Information: Neurochem. Res.; (United States), Vol. 8:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BRAIN
ONTOGENESIS
LIPOPROTEINS
BIOSYNTHESIS
LABELLING
MYELIN
CARBOXYLIC ACIDS
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BODY
CENTRAL NERVOUS SYSTEM
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RODENTS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550801 - Morphology- Tracer Techniques