Effects of neurotransmitters on calcium efflux from cultured glioma cells
The effects of various neurotransmitters and cyclic nucleotides on 45Ca2+ efflux in cultured human glioma cells were investigated. Glutamate and glycine, but not GABA, stimulated 45Ca2+ release from the cells. Stimulation of beta-adrenergic receptors but not alpha-adrenergic receptors also increased 45Ca2+ efflux. Cholinergic receptor stimulation by carbachol had the same effect. The stimulatory effect of carbachol was abolished in the presence of either atropine or hexamethonium. C-AMP and c-GMP increased the 45Ca2+ efflux, suggesting that these agents are involved in the transmitter-stimulated release of 45Ca2+ from the cell. Kinetic analysis of the efflux revealed four calcium compartments. The carbachol-stimulated efflux represented a net release of calcium and could be ascribed to the slowest compartment. The physiological role of the transmitter-stimulated calcium release is discussed in terms of calcium-regulated stimulus-response coupling in glial-neural interaction during excitation.
- Research Organization:
- Medical Research Centre, Polish Academy of Sciences, Warsaw
- OSTI ID:
- 5394232
- Journal Information:
- J. Neurosci. Res.; (United States), Vol. 6:3
- Country of Publication:
- United States
- Language:
- English
Similar Records
Prejunctional inhibition of norepinephrine release caused by acetylcholine in the human saphenous vein
Calcium permeability changes and neurotransmitter release in cultured rat brain neurons. I. Effects of stimulation on calcium fluxes
Related Subjects
CALCIUM 45
TRACER TECHNIQUES
CALCIUM IONS
BIOCHEMICAL REACTION KINETICS
TUMOR CELLS
PHYSIOLOGY
AMP
CELL CULTURES
COMPARTMENTS
GLIOMAS
RECEPTORS
STIMULI
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM ISOTOPES
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
NEOPLASMS
NUCLEI
NUCLEOTIDES
ORGANIC COMPOUNDS
RADIOISOTOPES
REACTION KINETICS
550201* - Biochemistry- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques
550301 - Cytology- Tracer Techniques