Studies on dibenzothiophene degrading bacteria

Conference · Sun Jan 01 00:00:00 EST 1989

OSTI ID:5392304

Key, D. H.; Willey, M. S.; Kase, R. S.; Ward, T. E.

We are studying the interaction of several bacterial isolates with dibenzothiophene (DBT), a sulfur-containing model compound similar to some of the sulfur-containing components of fossil fuels. The goal is to use these bacteria for microbiological coal desulfurization. Approximately 20 strains that metabolize DBT have been identified using an assay based on the oxidation/reduction indicator dye triphenyltetrazolium chloride (TTC). These isolates possess different types of interactions with DBT, based on the variety of physiological responses observed. The extent to which these isolates degrade DBT under specific conditions has been quantitated, using chloroform extraction of entire cultures followed by gas chromatographic analysis, and ranges from zero to about 60%. Some of the strains have been tentatively identified as Pseudomonas species. Studies of the metabolites produced from DBT by these isolates are in progress. 10 refs., 3 tabs.

• Research Organization: EG and G Idaho, Inc., Idaho Falls, ID (United States)
• Sponsoring Organization: DOE/FE
• DOE Contract Number: AC07-76ID01570
• OSTI ID: 5392304
• Report Number(s): EGG-M-89194; CONF-890582-3; ON: DE90001750
• Resource Relation: Conference: Biological processing of coal and coal-derived substances, Palo Alto, CA (USA), 16-17 May 1989
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
59 BASIC BIOLOGICAL SCIENCES
COAL
DESULFURIZATION
THIOPHENE
BIODEGRADATION
CULTURE MEDIA
DYES
INDICATORS
METABOLITES
PSEUDOMONAS
BACTERIA
CARBONACEOUS MATERIALS
CHEMICAL REACTIONS
DECOMPOSITION
ENERGY SOURCES
FOSSIL FUELS
FUELS
HETEROCYCLIC COMPOUNDS
MATERIALS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
010402* - Coal
Lignite
& Peat- Purification & Upgrading
550700 - Microbiology