Analysis of human muscle extracts by proton NMR
Perchloric acid extracts were prepared from pooled human muscle biopsies from patients diagnosed with scoliosis (SCOL) and cerebral palsy (CP). After neutralization with KOH and removal of perchlorate, the extracts were concentrated by freeze drying and dissolved in /sup 2/H/sub 2/O to contain 120 O.D. units at 280 nm per 0.5 ml. /sup 1/H NMR spectroscopy was performed with the 5 mm probe of a Varian XL300 instrument. Creatine, lactate, carnosine, and choline were the major resonances in the one-dimensional spectra of both extracts. With creatine as reference, 2.5-fold more lactate was found in SCOL than in CP, and a much smaller difference was also found in their carnosine content. Two-dimensional COSY comparison revealed several differences between the two extracts. Taurine, N-acetyl glutamate, glycerophosphoryl choline (or phosphoryl choline) and an unidentified spot were present only in the extract from SCOL but not in that from CP. On the other hand, aspartate, hydroxy-proline, carnitine and glycerophosphoryl ethanolamine were only present in CP but absent in SCOL. Alanine, cysteine, lysine and arginine appeared in both extracts without an apparent intensity difference.
- Research Organization:
- Univ. of Illinois, Chicago
- OSTI ID:
- 5391726
- Report Number(s):
- CONF-8604222-; TRN: 86-028448
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
MUSCLES
NMR SPECTRA
CHOLINE
CREATINE
DEUTERIUM
LACTIC ACID
NUCLEAR MAGNETIC RESONANCE
TRACER TECHNIQUES
WATER
ALCOHOLS
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
CARBOXYLIC ACIDS
DRUGS
HYDROGEN COMPOUNDS
HYDROGEN ISOTOPES
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAGNETIC RESONANCE
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
QUATERNARY COMPOUNDS
RESONANCE
SPECTRA
STABLE ISOTOPES
550600* - Medicine
550201 - Biochemistry- Tracer Techniques