Measurement of pectin methylation in plant cell walls

Journal Article · Sun Jan 01 00:00:00 EST 1984 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(84)90407-X· OSTI ID:5389764

McFeeters, R F; Armstrong, S A

A procedure was developed to measure the degree of pectin methylation in small samples of isolated cell walls from nonlignified plant tissues or pectin solutions. Galacturonic acid was determined colorimetrically with the 3,5-dimethylphenol reagent. Methylation was measured by base hydrolysis of galacturonic acid methyl esters, followed by gas chromatographic determination of released methanol. Estimates of the precision of analysis of pectin and cell wall samples were made. The coefficient of variation for estimates of the pectin esterification in cell walls isolated from 10-g samples of cucumber tissue ranged from 7.7 to 13.2%.

• Research Organization: North Carolina State Univ., Raleigh
• OSTI ID: 5389764
• Journal Information: Anal. Biochem.; (United States), Vol. 136:1, Issue 1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PECTINS
ABSORPTION SPECTROSCOPY
METHYLATION
CELL WALL
GAS CHROMATOGRAPHY
PLANT CELLS
BLOOD SUBSTITUTES
CARBOHYDRATES
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DRUGS
HEMATOLOGIC AGENTS
ORGANIC COMPOUNDS
POLYSACCHARIDES
SACCHARIDES
SEPARATION PROCESSES
SPECTROSCOPY
550200* - Biochemistry