Detection of early changes in lung cell cytology by flow-systems analysis techniques. [Rats]
Ongoing experiments designed to develop automated flow-analysis methods for assaying damage to pulmonary lavage cells in experimental animals exposed by inhalation to environmental pollutants are summarized. Pulmonary macrophages were characterized on their ability to phagocytize polystyrene latex fluorescent spheres. Lung cells consisting primarily of macrophages and leukocytes were analyzed for fluorescence (phagocytosis of spheres) and size using flow cytometric methods. Studies also concentrated on combining phagocytosis with other cellular parameters (DNA content, cell viability, and B-glucuronidase activity). As baseline studies are completed in normal animals, experimental animals will be exposed to gaseous and particulate environmental pollutants. (ERB
- Research Organization:
- Los Alamos Scientific Lab., NM (USA)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 5387977
- Report Number(s):
- LA-8302
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
54 ENVIRONMENTAL SCIENCES
AIR POLLUTION
BIOLOGICAL EFFECTS
LEUKOCYTES
ENZYME ACTIVITY
PHAGOCYTOSIS
MACROPHAGES
BENCH-SCALE EXPERIMENTS
CELL FLOW SYSTEMS
CYTOLOGICAL TECHNIQUES
DNA
EXPERIMENTAL DATA
EXPOSURE CHAMBERS
FLUORESCENCE
GLUCURONIDASE
GRAPHS
INHALATION
INTRATRACHEAL ADMINISTRATION
LATEX
MICROSPHERES
POLYSTYRENE
RATS
RESPIRATORY TRACT CELLS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DATA
DATA FORMS
ELASTOMERS
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
INFORMATION
INTAKE
LUMINESCENCE
MAMMALS
MATERIALS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PETROCHEMICALS
PETROLEUM PRODUCTS
PHAGOCYTES
PLASTICS
POLLUTION
POLYMERS
POLYOLEFINS
POLYVINYLS
RODENTS
RUBBERS
SOMATIC CELLS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)
550300 - Cytology
550200 - Biochemistry
500200 - Environment
Atmospheric- Chemicals Monitoring & Transport- (-1989)