Glyceraldehyde-3-phosphate dehydrogenase gene from Zymomonas mobilis: cloning, sequencing, and identification of promoter region
The gene encoding glyceraldehyde-3-phosphate dehydrogenase was isolated from a library of Zymomonas mobilis DNA fragments by complementing a deficient strain of Escherichia coli. It contained tandem promoters which were recognized by E. coli but appeared to function less efficiently than the enteric lac promoter in E. coli. The open reading frame for this gene encoded 337 amino acids with an aggregate molecular weight of 36,099 (including the N-terminal methionine). The primary amino acid sequence for this gene had considerable functional homology and amino acid identity with other eukaryotic and bacterial genes. Based on this comparison, the gap gene from Z. mobilis appeared to be most closely related to that of the thermophilic bacteria and to the chloroplast isozymes. Comparison of this gene with other glycolytic enzymes from Z. mobilis revealed a conserved pattern of codon bias and several common features of gene structure. A tentative transcriptional consensus sequence is proposed for Z. mobilis based on comparison of the five known promoters for three glycolytic enzymes.
- Research Organization:
- Univ. of Florida, Gainesville
- DOE Contract Number:
- FG05-86ER13575
- OSTI ID:
- 5382004
- Journal Information:
- J. Bacteriol.; (United States), Vol. 169:12
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cloning of the Zymomonas mobilis structural gene encoding alcohol dehydrogenase I (adhA): Sequence comparison and expression in Escherichia coli
Cloning and sequencing of the alcohol dehydrogenase II gene from zymomonas mobilis
Related Subjects
09 BIOMASS FUELS
OXIDOREDUCTASES
DNA SEQUENCING
DNA-CLONING
ZYMOMONAS MOBILIS
MOLECULAR BIOLOGY
AMINO ACID SEQUENCE
ESCHERICHIA COLI
GENE REPRESSORS
BACTERIA
CLONING
ENZYMES
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
550200* - Biochemistry
090222 - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
550700 - Microbiology