Growth factors with heparin binding affinity in human synovial fluid

Journal Article · Tue Dec 01 00:00:00 EST 1987 · Proc. Soc. Exp. Biol. Med.; (United States)

DOI:https://doi.org/10.3181/00379727-186-3-RC2· OSTI ID:5381602

Hamerman, D; Taylor, S; Kirschenbaum, I; Klagsbrun, M; Raines, E W; Ross, R; Thomas, K A

Synovial effusions were obtained from the knees of 15 subjects with joint trauma, menisceal or ligamentous injury, or osteoarthritis. Heparin-Sepharose affinity chromatography of these synovial fluids revealed, in general, three major peaks of mitogenic activity as measured by incorporation of /sup 3/H-thymidine into 3T3 cells. Gradient elution patterns showed activities at 0.5M NaCl, which is characteristic of platelet derived growth factor, and at 1.1 M NaCl and 1.6M NaCl, indicative of acidic and basic fibroblast growth factors, respectively. The identities of these mitogenic fractions were confirmed by specific immunologic and receptor-binding assays. The presence of platelet derived, acidic and basic fibroblast growth factors in the synovial fluid may contribute to wound healing in the arthritic joint.

• Research Organization: Albert Einstein College of Medicine, Bronx, NY (USA)
• OSTI ID: 5381602
• Journal Information: Proc. Soc. Exp. Biol. Med.; (United States), Vol. 186:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
SKELETAL DISEASES
PATHOGENESIS
WOUNDS
HEALING
AFFINITY
CHROMATOGRAPHY
FIBROBLASTS
HEPARIN
MAN
MITOGENS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
ANIMAL CELLS
ANIMALS
ANTICOAGULANTS
AZINES
BIOLOGICAL RECOVERY
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DISEASES
DRUGS
FUNCTIONS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
INJURIES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MUCOPOLYSACCHARIDES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYSACCHARIDES
PRIMATES
PROTEINS
PYRIMIDINES
REACTION KINETICS
RECOVERY
RIBOSIDES
SACCHARIDES
SEPARATION PROCESSES
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques