Direct radioimmunoassay (RIA) of salivary testosterone: correlation with free and total serium testosterone

Journal Article · Mon Aug 26 00:00:00 EDT 1985 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(85)90540-5· OSTI ID:5379790

Vittek, J; L'Hommedieu, D G; Gordon, G G; Rappaport, S C; Southren, A L

Simple and sensitive direct RIA for determination of salivary testosterone was developed by using RSL NOSOLVEX TM (125 1) kit produced by Radioassay System Laboratories (Carcon, California). In addition, a relationship between salivary and serum free and total testosterone concentrations was studied in randomly selected 45 healthy subjects, 5 females on oral contraceptive pills and 28 hypertensive patients on various treatment regimens. The lowest weight of testosterone detectable by the modified method was equivalent to 1 pg/ml of saliva, taking into account analytical variability. Intra- and interassay coefficients of variation were 5.09 +/- 2.7% and 8.2 +/- 5.9% respectively. Statistically significant correlations were found between salivary and serum free testosterone (r = 0.97) and salivary and serum total testosterone concentrations (r = 0.70 - 0.87). The exception to this was a group of hypertensive females in which no correlation (r = 0.14) between salivary and total serum testosterone was found. It is also of interest that, while salivary testosterone was significantly increased in subjects taking oral contraceptives and most of the hypertensive patients, the total serum testosterone concentration was in normal range. These findings suggest that the determination of salivary testosterone is a reliable method to detect changes in the concentration of available biologically active hormone in the circulation. 21 references, 4 figures, 1 table.

• Research Organization: New York Medical College, Valhalla
• OSTI ID: 5379790
• Journal Information: Life Sci.; (United States), Vol. 37:8
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
TESTOSTERONE
RADIOIMMUNOASSAY
PATIENTS
SALIVA
SALIVARY GLANDS
ANDROGENS
ANDROSTANES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
GLANDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
MATERIALS
ORGANIC COMPOUNDS
ORGANS
RADIOASSAY
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics