Induction and disappearance of DNA strand breaks in human peripheral blood lymphocytes and fibroblasts treated with methyl methanesulfonate

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(91)90157-P· OSTI ID:5375789

Boerrigter, M E.T.I.; Mullaart, E; Vijg, J ^[1]

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1. TNO Inst. of Experimental Gerontology, Rijswijk (Netherlands)

The induction and disappearance of DNA single-strand breaks (SSB) in human peripheral blood lymphocytes (PBL) and fibroblasts exposed to methyl methanesulfonate (MMS) were investigated by using the alkaline filter elution assay. In the two cell types, identical amounts of SSB were induced during a 45-minute treatment with a given dose of MMS. In quiescent PBL only 9{plus minus}4% (mean {plus minus} SD) of the induced SSB had disappeared at 1 hour after exposure, whereas in phytohemagglutinin-stimulated PBL, 23 {plus minus} 12% disappeared within the same repair period. The accumulation of SSB in PBL, but not in fibroblasts, during MMS exposure in the presence of the excision-repair inhibitor 1-{beta}-D-arabinofuranosylcytosine indicated the utilization of different repair pathways in these two cell types. The generally lower rate of disappearance of MMS-induced SSB in PBL as compared to fibroblasts correlated with an increased loss of cell viability, measured by determining the incorporation of ({sup 3}H)thymidine.

• OSTI ID: 5375789
• Journal Information: Experimental Cell Research; (United States), Vol. 192:1; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA ADDUCTS
MOLECULAR BIOLOGY
METHYL METHANESULFONATE
BIOLOGICAL EFFECTS
STRAND BREAKS
BIOLOGICAL REPAIR
ALKYLATING AGENTS
DNA
FIBROBLASTS
LYMPHOCYTES
THYMIDINE
TRITIUM COMPOUNDS
ADDUCTS
ANIMAL CELLS
AZINES
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ESTERS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
LEUKOCYTES
MATERIALS
MUTAGENS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PYRIMIDINES
REPAIR
RIBOSIDES
SOMATIC CELLS
SULFONIC ACID ESTERS
560300* - Chemicals Metabolism & Toxicology