Errors of utilizing carbon monoxide uptake to determine red cell mass

Conference · Mon Mar 11 00:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5372903

McNeil, J; Mundie, T; Ripple, G; Jackson, B; Martin, D ^[1]

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1. WRAIR, Washington, DC (United States)

Carbon monoxide (CO) has been used to approximate effective red cell mass (E{sub RCM}) utilizing the assumption that CO has a simple volume of distribution as carboxyhemoglobin (COHb). However, CO is known to bind to other heme molecules in vitro, and the extent of binding in vivo is unknown. E{sub RCM} was determined for six adult female splenectomized sheep using a CO containing re-breathing exposure system which allowed the animal to rapidly attain a steady state of COHb. Carbon dioxide was removed from the system and O{sub 2} was added as needed to maintain a constant system volume and O{sub 2} concentration. E{sub RCM} was calculated from measurements using a low, medium and high CO concentration. Results were compared with measurements of RCM done using the chromium 51 dilution method. The mean RCM ({plus minus} S.D.) was 1.87 {plus minus} 0.27 liters; while the mean E{sub RCM} ({plus minus} S.D.) was 2.56 {plus minus} 0.40. The E{sub RCM} was found to be 26.3% greater than the RCM suggesting significant tissue binding and that CO should not be used to estimate Red Cell Mass due to this binding.

• OSTI ID: 5372903
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARBON MONOXIDE
UPTAKE
ERYTHROCYTES
MEASURING METHODS
CHEMICAL BONDS
DATA COVARIANCES
METABOLISM
SHEEP
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
DOMESTIC ANIMALS
MAMMALS
MATERIALS
OXIDES
OXYGEN COMPOUNDS
RUMINANTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology