Enzyme-based detection of chlorinated hydrocarbons in water. Report for October 1982-July 1983

Offenhartz, B H; Lefko, J L

Title: Enzyme-based detection of chlorinated hydrocarbons in water. Report for October 1982-July 1983

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Abstract

An enzyme-based approach for detecting hazardous levels of high-molecular-weight chlorinated hydrocarbons in natural waters was explored. An extensive review of the literature indicated that the enzymes, lactate dehydrogenase, carbonic anhydrase, hexokinase, phosphorylase and an ATPase are suitable for field method development. Lactate dehydrogenase (LDH) was chosen to develop a method that promises rapid, reliable, and cost-effective detection. The LDH-catalyzed reaction is used to detect chlorinated hydrocarbons, which reduce the rate of the reaction by reversibly inhibiting the enzyme. The analysis uses pH detection and takes 5 minutes. So far, the LDH method has been used to detect aldrin, toxaphene, DDT, PCBs, pentachlorophenol and 2,4,5,-T at the parts per million level. A review of the literature suggests that most organochlorine pesticides, PCBs, polychlorinated phenols and chlorophenoxy derivatives can be detected by the LDH method. Preliminary investigations of potential interferants suggest that the LDH method selectively detects chlorinated hydrocarbons. Work was begun on immobilizing the enzyme for incorporation in water monitor and field detector designs. The LDH method shows potential for use in field devices and for processing extracts of soils and wastes, as well as water samples. These method adaptations are recommended for future work.

Authors:: Offenhartz, B H; Lefko, J L

Publication Date:: Mon Apr 01 00:00:00 EST 1985

Research Org.:: JRB Associates, Inc., McLean, VA (USA)

OSTI Identifier:: 5371351

Report Number(s):: PB-85-191716/XAB

Resource Type:: Technical Report

Resource Relation:: Other Information: Also published as B and M Technological Services, Inc., Cambridge, MA. rept. no. B/M-EPA-82-01

Country of Publication:: United States

Language:: English

Subject:: 54 ENVIRONMENTAL SCIENCES; ENZYME ACTIVITY; HAZARDOUS MATERIALS; ORGANIC CHLORINE COMPOUNDS; LACTATE DEHYDROGENASE; WATER POLLUTION; DETECTION; CATALYSIS; FIELD TESTS; MONITORS; PESTICIDES; SAMPLING; WATER QUALITY; ENVIRONMENTAL QUALITY; ENZYMES; HEMIACETAL DEHYDROGENASES; MATERIALS; MEASURING INSTRUMENTS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; OXIDOREDUCTASES; POLLUTION; TESTING; 520200* - Environment, Aquatic- Chemicals Monitoring & Transport- (-1989)

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                    Offenhartz, B H, and Lefko, J L. Enzyme-based detection of chlorinated hydrocarbons in water. Report for October 1982-July 1983.  United States: N. p., 1985. 
        Web.

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                    Offenhartz, B H, & Lefko, J L. Enzyme-based detection of chlorinated hydrocarbons in water. Report for October 1982-July 1983.  United States.

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                    Offenhartz, B H, and Lefko, J L. 1985.  
        "Enzyme-based detection of chlorinated hydrocarbons in water. Report for October 1982-July 1983".  United States.

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@article{osti_5371351,

  title        = {Enzyme-based detection of chlorinated hydrocarbons in water. Report for October 1982-July 1983},

  author       = {Offenhartz, B H and Lefko, J L},

  abstractNote = {An enzyme-based approach for detecting hazardous levels of high-molecular-weight chlorinated hydrocarbons in natural waters was explored. An extensive review of the literature indicated that the enzymes, lactate dehydrogenase, carbonic anhydrase, hexokinase, phosphorylase and an ATPase are suitable for field method development. Lactate dehydrogenase (LDH) was chosen to develop a method that promises rapid, reliable, and cost-effective detection. The LDH-catalyzed reaction is used to detect chlorinated hydrocarbons, which reduce the rate of the reaction by reversibly inhibiting the enzyme. The analysis uses pH detection and takes 5 minutes. So far, the LDH method has been used to detect aldrin, toxaphene, DDT, PCBs, pentachlorophenol and 2,4,5,-T at the parts per million level. A review of the literature suggests that most organochlorine pesticides, PCBs, polychlorinated phenols and chlorophenoxy derivatives can be detected by the LDH method. Preliminary investigations of potential interferants suggest that the LDH method selectively detects chlorinated hydrocarbons. Work was begun on immobilizing the enzyme for incorporation in water monitor and field detector designs. The LDH method shows potential for use in field devices and for processing extracts of soils and wastes, as well as water samples. These method adaptations are recommended for future work.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/5371351},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Mon Apr 01 00:00:00 EST 1985},

  month        = {Mon Apr 01 00:00:00 EST 1985}

}

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