Lactoperoxidase-catalyzed iodination of membrane proteins in normal and neoplastic epidermal cells
Cell surface proteins of normal human, mouse, and rat cells in primary culture, of human basal cell carcinoma, and of carcinogen-transformed cell lines were examined by lactoperoxidase-catalyzed iodination. Autoradiography was used to record the distribution of label in the polypeptide subunits separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. There was no significant difference in the results for normal cells of human, mouse, and rat. On the other hand, carcinogen-transformed mouse cells had many more labeled polypeptide bands of widely distributed molecular weights. The iodination profiles from human basal cell carcinoma cells were much more akin to those from normal cells than to those from carcinogen-transformed cells. Treatment of iodinated cells with proteolytic enzymes visibly altered the polypeptide bands.
- Research Organization:
- Department of Dermatology, University of Texas, Medical Branch Galveston
- OSTI ID:
- 5368052
- Journal Information:
- J. Invest. Dermatol.; (United States), Vol. 78:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CARCINOMAS
PATHOLOGY
TUMOR CELLS
BIOCHEMISTRY
AUTORADIOGRAPHY
CELL CULTURES
CELL MEMBRANES
COMPARATIVE EVALUATIONS
IODINATION
LABELLED COMPOUNDS
MAN
MICE
MOLECULAR WEIGHT
PEROXIDASES
PROTEINS
RATS
ANIMAL CELLS
ANIMALS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHEMISTRY
DISEASES
ENZYMES
HALOGENATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAMMALS
MEMBRANES
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PORPHYRINS
PRIMATES
RODENTS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques