Interleukin-2 (rIL-2)-induced lymphokine-activated killer (LAK) cells and their precursors express the VGO1 antigen

Journal Article · Sat Jul 01 00:00:00 EDT 1989 · Journal of Clinical Immunology; (USA)

DOI:https://doi.org/10.1007/BF00918668· OSTI ID:5364599

Denegri, J F; Peterson, J; Tilley, P ^[1]

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1. Vancouver General Hospital, B.C. (Canada)

Precursor and effector cells of recombinant interleukin-2 (r-IL-2)-induced lymphokine-activated killer (LAK) activity were investigated for their expression of VGO1. Peripheral blood lymphocytes (PBL) from normal donors were purified and separated in a FACS 420 into VGO1+- and VGO1- cell fractions before and after culture for 96 hr with 100 U/ml of r-IL-2. Their lytic activity against K 562 and Daudi cells was measured in a 51Cr release assay. The majority, if not all, of the LAK effector and precursor cells was VGO1+ lymphocytes. The expression of VGO1 by LAK precursor cells remained stable under the culture conditions used in our experiments. VGO1- lymphocytes cultured with r-IL-2 demonstrated neither LAK-induced activity nor expression of VGO1 antigen.

• OSTI ID: 5364599
• Journal Information: Journal of Clinical Immunology; (USA), Vol. 9:4; ISSN 0271-9142
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
LYMPHOCYTES
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550201* - Biochemistry- Tracer Techniques