Excision repair and patch size in UV-irradiated bacteriophage T4

Journal Article · Sun Nov 01 00:00:00 EST 1981 · J. Virol.; (United States)

OSTI ID:5363375

Yarosh, D B; Rosenstein, B S; Setlow, R B

We determined the average size of excision repair patches in repair of UV lesions in bacteriophage T4 by measuring the photolysis of bromodeoxyuridine incorporated during repair. The average patch was small, approximately four nucleotides long. In control experiments with the denV1 excision-deficient mutant, we encountered an artifact, a protein(s) which remained bound to phenol-extracted DNA and prevented nicking by the UV-specific endonucleases of Micrococcus luteus and bacteriophage T4.

• Research Organization: Nucleic Acids Section, Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda
• OSTI ID: 5363375
• Journal Information: J. Virol.; (United States), Vol. 40:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BACTERIOPHAGES
BIOLOGICAL RADIATION EFFECTS
DNA
BIOLOGICAL REPAIR
BIOCHEMISTRY
BUDR
PHOTOLYSIS
ULTRAVIOLET RADIATION
ANTIMETABOLITES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BROMOURACILS
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PHOTOCHEMICAL REACTIONS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
URACILS
VIRUSES
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