Nuclear Medicine Program progress report for quarter ending September 30, 1989

Technical Report · Fri Dec 01 00:00:00 EST 1989

DOI:https://doi.org/10.2172/5358657· OSTI ID:5358657

Knapp, Jr., F. F.; Ambrose, K. R.; Callahan, A. P.; Allred, J. F.; Blystone, S. L.; Hasan, A.; McPherson, D. W.; Srivastava, P. C.; Lambert, C. R.; Lambert, S. J.; Rice, D. E.

In this report, an evaluation of the effects of albumin and albumin plus sodium palmitate in the phosphate buffer perfusate on the relative of unmetabolized fatty acid and the unknown metabolite(s) from isolated rat hearts administered 15-(p-(I-125)iodophenyl)-3-(R, S)-methylpentadecanoic acid (BMIPP) is described. Earlier studies had demonstrated the presence of a major unidentified polar radioactive component in the outflow of hearts injected with BMIPP and perfused with the traditional Krebs-Henseleit (KH) buffer, which does not contain albumin. The current studies were performed with KH buffer containing either albumin (BSA) or albumin and palmitate (BSA/PAL) to assess the relative loss of the metabolite and unmetabolized BMIPP from the perfused hearts. The results demonstrated that in the presence of albumin both the unidentified material and BMIPP are present in the outflow (i.e., 5 min perfusate buffer, % BMIPP: KH, 3%; KH + BSA, 10%; KH + BSA/PAL, 41%). These results demonstrate that BMIPP is a major radioactive component in the outflow of isolated hearts using a perfusate containing BSA and palmitate and, more importantly, suggest for the first time that the slow myocardial wash-out observed in humans after administration of (I-123)BMIPP probably represents loss of both unmetabolized BMIPP and the unidentified metabolite. Coronary sinus sampling studies with dogs are now in progress to relate the relative contribution of these two components to the release of radioactivity from the heart. Also in this report, our population experience for several radioisotopes being used by the ORNL Nuclear Medicine Program is summarized.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: DOE/ER
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5358657
• Report Number(s): ORNL/TM-11377; ON: DE90004434
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
MONOCARBOXYLIC ACIDS
IODINATION
RADIOPHARMACEUTICALS
SYNTHESIS
HEART
NUCLEAR MEDICINE
PROGRESS REPORT
PURIFICATION
RADIOCHEMISTRY
RATS
SALES
ANIMALS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
CHEMISTRY
DOCUMENT TYPES
DRUGS
HALOGENATION
LABELLED COMPOUNDS
MAMMALS
MEDICINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RODENTS
VERTEBRATES
550600* - Medicine
550500 - Metabolism