Molecular basis for the CAT-2 null phenotype in maize

Journal Article · Fri Jan 01 00:00:00 EST 1988 · Genetics; (United States)

OSTI ID:5357707

Bethards, L A; Scandalios, J G

Previous reports have described several maize lines whose developmental patterns of catalase gene expression vary from the typical maize line, W64A. Among these variants are the lines A16 and A338, both found to be null for the CAT-2 protein. Identification of a third CAT-2 null line, designated A340, is described. RNA blots and S1 nuclease protection analysis, using (/sup 32/P)-labeled dCTP, indicate that all three CAT-2 null lines produce a similarly shortened Cat2 transcript. The molecular basis for this aberrant Cat2 transcript is discussed.

• Research Organization: North Carolina State Univ., Raleigh
• OSTI ID: 5357707
• Journal Information: Genetics; (United States), Vol. 118:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CATALASE
GENE REGULATION
MAIZE
ENZYME ACTIVITY
MOLECULAR BIOLOGY
ALCOHOL DEHYDROGENASE
ELECTROPHORESIS
NUCLEOTIDES
PHENOTYPE
PHOSPHORUS 32
PROTEINS
SUPEROXIDE DISMUTASE
TRANSCRIPTION
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CEREALS
DAYS LIVING RADIOISOTOPES
ENZYMES
GRASS
HEMIACETAL DEHYDROGENASES
ISOTOPES
LIGHT NUCLEI
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
PHOSPHORUS ISOTOPES
PLANTS
RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques