Early estrogen-induced metabolic changes and their inhibition by actinomycin D and cycloheximide in human breast cancer cells: sup 31 P and sup 13 C NMR studies

Journal Article · Sat Jul 01 00:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.14.5585· OSTI ID:5351854

Neeman, M; Degani, H ^[1]

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1. Weizmann Institute of Science, Rehovot (Israel)

Metabolic changes following estrogen stimulation and the inhibition of these changes in the presence of actinomycin D and cycloheximide were monitored continuously in perfused human breast cancer T47D clone 11 cells with {sup 31}P and {sup 13}C NMR techniques. The experiments were performed by estrogen rescue of tamoxifen-treated cells. Immediately after perfusion with estrogen-containing medium, a continuous enhancement in the rates of glucose consumption, lactate production by glycolysis, and glutamate synthesis by the Krebs cycle occurred with a persistent 2-fold increase at 4 hr. Pretreatment with either actinomycin D or cycloheximide, at concentrations known to inhibit mRNA and protein synthesis, respectively, and simultaneous treatment with estrogen and each inhibitor prevented the estrogen-induced changes in glucose metabolism. This suggested that the observed estrogen stimulation required synthesis of mRNA and protein. These inhibitors also modulated several metabolic activities that were not related to estrogen stimulation. The observed changes in the in vivo kinetics of glucose metabolism may provide a means for the early detection of the response of human breast cancer cells to estrogen versus tamoxifen treatment.

• OSTI ID: 5351854
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:14; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ACTINOMYCIN
BIOLOGICAL EFFECTS
CYCLOHEXIMIDE
ESTRADIOL
METABOLISM
TUMOR CELLS
NUCLEAR MAGNETIC RESONANCE
CARBON 13
CHEMICAL SHIFT
MAMMARY GLANDS
MAN
MESSENGER-RNA
NMR SPECTRA
OVERHAUSER EFFECT
PHOSPHORUS 31
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BODY
CARBON ISOTOPES
DRUGS
ESTRANES
ESTROGENS
EVEN-ODD NUCLEI
FUNGICIDES
GLANDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PESTICIDES
PHOSPHORUS ISOTOPES
PRIMATES
RESONANCE
RNA
SPECTRA
STABLE ISOTOPES
STEROID HORMONES
STEROIDS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics