Comparison between immobilized Kluyveromyces fragilis and saccharomyces cerevisiae coimmobilized with. beta. -galactosidase, with respect to continuous ethanol production from concentrated whey permeate

Journal Article · Sat Jun 01 00:00:00 EDT 1985 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260270627· OSTI ID:5351558

Hahn-Haegerdal, B

Kluyveromyces fragilis immobilized in calcium alginate gel was compared to Saccharomyces cerevisiae coimmobilized with ..beta..-galactosidase, for continuous ethanol production from whey permeate in packed-bed-type columns. Four different whey concentrations were studied, equivalent to 4.5, 10, 15 and 20% lactose, respectively. In all cases the coimmobilized preparation produced more ethanol than Kluyveromyces fragilis. The study went on for more than 5 weeks. Kluyveromyces fragilis showed a decline in activity after 20 days, while the coimmobilized preparation was stable during the entire investigation. Under experimental conditions theoretical yields of ethanol were obtained from 4.5 and 10% lactose substrates with the coimmobilized system. Using 15% lactose substrate, theoretical yields were only obtained when a galactose-adapted immobilized Saccharomyces cerevisiae column was run in series with the coimmobilized column. Then a maximum of 71 g/l ethanol was produced with a productivity of 2.5 g/l hour. The coimmobilized column alone gave a maximum ethanol concentration of 52 g/l with a productivity of 4.5 g/l hour, whereas immobilized Kluyveromyces fragilis only produced 13 g/l ethanol with a productivity of 1.1 g/l hour. It was not possible to obtain theoretical yields of ethanol from the highest substrate concentration. 13 references.

• Research Organization: Lund Univ. (Sweden)
• OSTI ID: 5351558
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 27:6
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
ETHANOL
PRODUCTION
SACCHAROMYCES CEREVISIAE
CONTINUOUS CULTURE
ENZYME ACTIVITY
WHEY
FERMENTATION
YEASTS
BIOREACTORS
COMPARATIVE EVALUATIONS
GALACTOSIDASE
IMMOBILIZED CELLS
IMMOBILIZED ENZYMES
LACTOSE
PACKED BED
PERFORMANCE TESTING
ALCOHOLS
BIOCONVERSION
CARBOHYDRATES
DISACCHARIDES
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
HYDROXY COMPOUNDS
INDUSTRIAL WASTES
MICROORGANISMS
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SACCHAROMYCES
TESTING
WASTES
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