Variation in the human lymphocyte sister chromatid exchange frequency as a function of time: results of daily and twice-weekly sampling
The variation in lymphocyte sister chromatid exchange (SCE) frequency was investigated in healthy nonsmokers who were not taking any medication. Two separate studies were undertaken. In the first, blood was drawn from four women twice a week for 8 weeks. These donors recorded the onset and termination of menstruation and times of illness. In the second study, blood was obtained from two women and two men for 5 consecutive days on two separate occasions initiated 14 days apart. Analysis of the mean SCE frequencies in each study indicated that significant temporal variation occurred in each donor, and that more variation occurred in the longer study. Some of the variation was found to be associated with the menstrual cycle. In the daily study, most of the variation appeared to be random, but occasional day-to-day changes occurred that were greater than those expected by chance. To determine how well a single SCE sample estimated the pooled mean for each donor in each study, the authors calculated the number of samples that encompassed that donor's pooled mean within 1 or more standard errors. For both studies, about 75% of the samples encompassed the pooled mean within 2 standard errors. An analysis of high-frequency cells (HFCs) was also undertaken. The results for each study indicate that the proportion of HFCs, compared with the use of Fisher's Exact test, is significantly more constant than the means, which were compared by using the t-test. These results coupled with our previous work suggest that HFC analysis may be the method of choice when analyzing data from human population studies.
- Research Organization:
- Lawrence Livermore National Lab., CA
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 5348505
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 10:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LYMPHOCYTES
SAMPLING
MUTATION FREQUENCY
DAILY VARIATIONS
MONTHLY VARIATIONS
BLOOD
CARCINOGEN SCREENING
HUMAN POPULATIONS
MEN
MUTAGEN SCREENING
SISTER CHROMATID EXCHANGES
WOMEN
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD CELLS
BODY FLUIDS
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
FEMALES
LEUKOCYTES
MALES
MAMMALS
MAN
MATERIALS
MUTATIONS
POPULATIONS
PRIMATES
SCREENING
SOMATIC CELLS
VARIATIONS
VERTEBRATES
550400* - Genetics