Simplified method for DNA and protein staining of human hematopoietic cell samples. [Cell flow systems]

Journal Article · Thu Jan 01 00:00:00 EST 1981 · Cytometry (Baltimore); (United States)

OSTI ID:5348342

Crissman, H A ^[1]; Egmond, J V; Holdrinet, R S; Pennings, A; Haanen, C

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1. Los Alamos National Lab., NM

A rapid reproducible method yielding high resolution analysis of DNA and protein in human hematopoietic cell samples has been developed by modification of the propidium iodide and fluorescein isothiocyanate procedure. Cell staining involves sequential addition of each reagent (RNase, fluorescein isothiocyanate and propidium iodide) to ethanol-fixed cells and requires no centrifugation steps. Stained cells are analyzed in the reagent solutions. Analysis of bone marrow samples from multiple myeloma patients showed mixed normal and aneuploid populations with a major portion of the aneuploid cells having a significantly higher protein content. This approach permitted differential cell cycle analysis of normal and the aneuploid populations.

• OSTI ID: 5348342
• Journal Information: Cytometry (Baltimore); (United States), Vol. 2:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL CYCLE
CHEMICAL ANALYSIS
CELL FLOW SYSTEMS
DIAGNOSTIC USES
DNA
PROTEINS
ANEUPLOIDY
ANIMAL CELLS
BLOOD FORMATION
BONE MARROW CELLS
NEOPLASMS
PATIENTS
PHOTOMETRY
CONNECTIVE TISSUE CELLS
DISEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLOIDY
SOMATIC CELLS
USES
550200* - Biochemistry
550300 - Cytology