Cold-induced changes in amphibian oocytes

Journal Article · Tue Aug 01 00:00:00 EDT 1989 · Experimental Cell Research; (USA)

DOI:https://doi.org/10.1016/0014-4827(89)90410-2· OSTI ID:5346705

Angelier, N; Moreau, N A; N'Da, E A; Lautredou, N F ^[1]

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1. Centre de Biologie Cellulaire, Ivry-sur-Seine (France)

Female Pleurodeles waltl newts (Amphibia, urodele), usually raised at 20 degrees C, were submitted to low temperatures; oocytes responded to this cold stress by drastic changes both in lampbrush chromosome structure and in protein pattern. Preexisting lateral loops of lampbrush chromosomes were reduced in size and number, while cold-induced loops which were tremendously developed, occurred on defined bivalents of the oocyte at constant, reproducible sites. A comparison of protein patterns in control and stressed oocytes showed two main differences: in stressed oocytes, overall protein synthesis was reduced, except for a set of polypeptides, the cold-stress proteins; second, there was a striking inversion of the relative amount of beta- and gamma-actin found in the oocyte nucleus before and after cold stress. Whereas beta-actin was the predominant form in control oocytes, gamma-actin became the major form in stressed oocytes.

• OSTI ID: 5346705
• Journal Information: Experimental Cell Research; (USA), Vol. 183:2; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
OOCYTES
SENSITIVITY
SALAMANDERS
TEMPERATURE EFFECTS
BIOLOGICAL STRESS
CHROMOSOMES
FEMALES
IN VITRO
PROTEINS
TWO-DIMENSIONAL ELECTROPHORESIS
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
ELECTROPHORESIS
GERM CELLS
ORGANIC COMPOUNDS
VERTEBRATES
560200* - Thermal Effects