Modulation of the expression of chondroitin sulfate proteoglycan in stimulated human monocytes

Journal Article · Tue Sep 05 00:00:00 EDT 1989 · Journal of Biological Chemistry; (USA)

OSTI ID:5345796

Uhlin-Hansen, L; Eskeland, T; Kolset, S O ^[1]

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1. Univ. of Tromso (Norway)

Proteoglycan biosynthesis was studied in human monocytes and monocyte-derived macrophages (MDM) after exposure to typical activators of the monocyte/macrophage system: interferon-gamma (IFN-gamma), lipopolysaccharide (LPS), and phorbol 12-myristate 13-acetate (PMA). By morphological examination, both monocytes and MDM were stimulated by these activators. Treatment with IFN-gamma resulted in a slight decrease in the expression of (35S)chondroitin sulfate proteoglycan (CSPG) in both monocytes and MDM, whereas LPS treatment increased the (35S)CSPG expression 1.8 and 2.2 times, respectively. PMA, in contrast, decreased the CSPG expression 0.4 times in monocytes, whereas MDM were stimulated to increase the biosynthesis 1.9 times. An increase in the sulfate density of the chondroitin sulfate chains was evident following differentiation of monocytes into MDM due to the expression of disulfated disaccharide units of the chondroitin sulfate E type (CS-E). However, monocytes exposed to PMA did also express disaccharides of the chondroitin sulfate E type. Furthermore, the expression of CS-E in MDM was increased 2 times following PMA treatment. An inactive phorbol ester, phorbol 12,13-diacetate, did not affect the expression of CS-E in either monocytes or MDM when compared with control cultures, suggesting that protein kinase C-dependent signal pathways may be involved in the regulation of sulfation of CSPG. Exposure to LPS or IFN-gamma did not lead to any changes in the sulfation of the chondroitin sulfate chains.

• OSTI ID: 5345796
• Journal Information: Journal of Biological Chemistry; (USA), Vol. 264:25; ISSN 0021-9258
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
GLYCOPROTEINS
BIOSYNTHESIS
INTERFERON
BIOLOGICAL EFFECTS
LIPOPOLYSACCHARIDES
PHORBOL ESTERS
BIOLOGICAL PATHWAYS
CELL CULTURES
CHONDROITIN
MACROPHAGES
MAN
MONOCYTES
SULFATES
SULFUR 35
TRACER TECHNIQUES
AMINES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARCINOGENS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ESTERS
EVEN-ODD NUCLEI
GROWTH FACTORS
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LIPIDS
LYMPHOKINES
MAMMALS
MATERIALS
MITOGENS
MUCOPOLYSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHAGOCYTES
POLYSACCHARIDES
PRIMATES
PROTEINS
RADIOISOTOPES
SACCHARIDES
SOMATIC CELLS
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES
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