Analytical scanning electron microscopy and x-ray microdiffractometry of renal calculi using etched plastic sections
Journal Article
·
· Scanning Electron Microsc.; (United States)
OSTI ID:5345700
We describe the technique and illustrate results of renal calculi analyses utilizing correlative analytical scanning electron microscopy and x-ray microdiffractometry on the same 100 micrometer Spurr embedded sections. Essential to the technique is the employment of an extremely sensitive x-ray microdiffractometer, and for morphological analysis, pretreatment of the sections with a sodium ethoxide etching solution. This technique facilitates more precise identification of crystalline constituents especially calcium apatite and magnesium ammonium phosphate hexahydrate (struvite). Further, the technique permits detection and mapping of both crystalline and amorphous constituents within calculi.
- Research Organization:
- Department of Pathology, Mount Sinai Hospital, Toronto, Canada
- OSTI ID:
- 5345700
- Journal Information:
- Scanning Electron Microsc.; (United States), Vol. Pt 3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
CALCULI
ELECTRON MICROPROBE ANALYSIS
SCANNING ELECTRON MICROSCOPY
CALCIUM COMPOUNDS
KIDNEYS
OXALATES
SODIUM OXIDES
ALKALI METAL COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
BODY
CARBOXYLIC ACID SALTS
CHALCOGENIDES
CHEMICAL ANALYSIS
ELECTRON MICROSCOPY
MICROANALYSIS
MICROSCOPY
ORGANS
OXIDES
OXYGEN COMPOUNDS
SODIUM COMPOUNDS
550602* - Medicine- External Radiation in Diagnostics- (1980-)
550900 - Pathology
59 BASIC BIOLOGICAL SCIENCES
CALCULI
ELECTRON MICROPROBE ANALYSIS
SCANNING ELECTRON MICROSCOPY
CALCIUM COMPOUNDS
KIDNEYS
OXALATES
SODIUM OXIDES
ALKALI METAL COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
BODY
CARBOXYLIC ACID SALTS
CHALCOGENIDES
CHEMICAL ANALYSIS
ELECTRON MICROSCOPY
MICROANALYSIS
MICROSCOPY
ORGANS
OXIDES
OXYGEN COMPOUNDS
SODIUM COMPOUNDS
550602* - Medicine- External Radiation in Diagnostics- (1980-)
550900 - Pathology