Characterization of foot-and-mouth disease virus gene products with antisera against bacterially synthesized fusion proteins

Journal Article · Sat Mar 01 00:00:00 EST 1986 · J. Virol.; (United States)

OSTI ID:5331904

Strebel, K; Beck, E; Strohmaier, K; Schaller, H

Defined segments of the cloned foot-and-mouth disease virus genome corresponding to all parts of the coding region were expressed in Escherichia coli as fusions to the N-terminal part of the MS2-polymerase gene under the control of the inducible lambdaPL promoter. All constructs yielded large amounts of proteins, which were purified and used to raise sequence-specific antisera in rabbits. These antisera were used to identify the corresponding viral gene products in /sup 35/S-labeled extracts from foot-and-mouth disease virus-infected BHK cells. This allowed us to locate unequivocally all mature foot-and-mouth disease virus gene products in the nucleotide sequence, to identify precursor-product relationships, and to detect several foot-and mouth disease virus gene products not previously identified in vivo or in vitro.

• Research Organization: Universitaet Heidelberg, West Germany
• OSTI ID: 5331904
• Journal Information: J. Virol.; (United States), Vol. 57:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CLONE CELLS
AMINO ACID SEQUENCE
GENES
CHEMICAL COMPOSITION
PROTEINS
IMMUNE REACTIONS
PURIFICATION
ESCHERICHIA COLI
GENOME MUTATIONS
LABELLED COMPOUNDS
PRECURSOR
SULFUR 35
TRACER TECHNIQUES
VIRAL DISEASES
VIRUSES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CULTURES
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
MOLECULAR STRUCTURE
MUTATIONS
NUCLEI
ORGANIC COMPOUNDS
PARASITES
RADIOISOTOPES
SULFUR ISOTOPES
550401* - Genetics- Tracer Techniques