Use of acetimidation in the NMR identification of neurophysin lysine protons
Acetimidation of the two lysine residues of neurophysin (NP) results in localized changes in the proton magnetic resonance spectrum, allowing identification of lysine side-chain resonances. Neither peptide-binding nor protein self-association appeared to be significantly altered by acetimidation. Additionally, no significant effect of either peptide-binding or self-association on lysine epsilon-CH/sub 2/ protons was seen. However, dimerization-induced NMR changes in the 1.6-1.8 ppm region, associated with lysine ..beta..,..gamma..,sigma protons, were altered in the acetimidated protein. In particular, while the spectrum of the acetimidated NP monomer was almost identical to that of the native protein, a shoulder at 1.72 ppm in the native protein dimer was shifted upfield in the modified dimer. Additionally the direction of NMR shifts in the 1.6-1.8 ppm region normally associated with peptide binding to the NP dimer appeared to be reversed in the acetimidated protein. Binding-induced and dimerization-induced changes in all other regions of the spectrum were identical in the native and modified proteins. These results suggest that one or both NP lysine residues may be near the dimer subunit interface and indicate an effect of peptide-binding on lysine side-chain environment.
- Research Organization:
- Cornell Univ. Medical College, New York, NY
- OSTI ID:
- 5326579
- Report Number(s):
- CONF-8606151-; TRN: 86-031398
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
LYSINE
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
PEPTIDES
BIOCHEMICAL REACTION KINETICS
PROTONS
RESIDUES
AMINO ACIDS
BARYONS
CARBOXYLIC ACIDS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
KINETICS
MAGNETIC RESONANCE
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
RESONANCE
550201* - Biochemistry- Tracer Techniques
550600 - Medicine