Alteration of macrophage membrane lipids following processing of bacterial peptidoglycan
As part of the continuing investigation into the role played by macrophages in antigen presentation and bacterial adjuvant activation, the authors have examined the metabolites produced by macrophages after encounter with peptidoglycan. Peptidoglycan was chosen because it contains N-acetyl-muramyl-L-alanyl-D-isoglutamine (muramyl dipeptide), a known adjuvant whose primary target cell is the macrophage. In previous work, the authors established that a series of muramyl dipeptide-like glycopeptides was released into the medium following phagocytosis of peptidoglycan by a macrophage cell line. Here the authors report on the finding that, additionally, a membrane lipid has been covalently altered by the addition of a peptidoglycan fragment. Bacillus subtilis cell walls which had been radiolabeled in their muramic acid, glucosamine and alanine residues, were incubated with the murine macrophage cell line RAW264. Using standard lipid extraction procedures, a lipid was isolated and found to contain equal molar ratios of alanine, glutamic acid and diaminopimelic acid. Since lipidated peptidoglycan peptides have been shown to be immunoactivators, the isolated lipid derivative may serve as a signal for interactions with other lymphocytes.
- Research Organization:
- Univ. of Minnesota, Minneapolis
- OSTI ID:
- 5325863
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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LIPIDS
MOLECULAR STRUCTURE
MACROPHAGES
BIOLOGICAL FUNCTIONS
ANTIGENS
BACILLUS SUBTILIS
CELL MEMBRANES
CELL WALL
COVALENCE
LABELLED COMPOUNDS
METABOLITES
PEPTIDES
PHAGOCYTOSIS
RADIOISOTOPES
TRACER TECHNIQUES
ULTRASTRUCTURAL CHANGES
ANIMAL CELLS
BACILLUS
BACTERIA
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
FUNCTIONS
ISOTOPE APPLICATIONS
ISOTOPES
MEMBRANES
MICROORGANISMS
MORPHOLOGICAL CHANGES
ORGANIC COMPOUNDS
PHAGOCYTES
PROTEINS
SOMATIC CELLS
550201* - Biochemistry- Tracer Techniques