Molecular and cellular properties of human neutrophil (N) receptors for leukotriene B/sub 4/ (LTB/sub 4/)

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5325822

Goldman, D W; Gifford, L A; Marotti, T; Chernov-Rogan, T; Goetzl, E J

Human Ns express two sets of stereospecific plasma membrane receptors for the potent chemotactic factor LTB/sub 4/. Chemotaxis and increased adherence of Ns are mediated by a mean of 4400 high-affinity receptors per N with a mean dissociation constant (Kd) of 0.39 nM, whereas lysosomal enzyme release and superoxide generation are transduced by 270,000 low-affinity receptor per N with a Kd of 62 nM. The high-affinity receptors are down-regulated selectively by prior exposure of Ns to LTB/sub 4/ and require guanine nucleotide-binding proteins for optimal expression and signal transduction. Affinity-radiolabeling and solubilization of LTB/sub 4/ receptors has permitted isolation and fragmentation of the principal receptor protein in quantities sufficient for structural studies. Rabbit anti-idiotypic antibodies to a mouse IgG/sub 2b/ monoclonal anti-LTB/sub 4/ with a binding stereospecificity identical to that of N receptors for LTB/sub 4/, blocks the binding of (/sup 3/H)LTB/sub 4/ to N receptors. Anti-idiotypic antibodies to LTB/sub 4/ receptors interact perferentially with the high-affinity receptors, elicit N functional responses, and suppress selectively the stimulatory effects of LTB/sub 4/ on N chemotaxis. Thus, techniques have been developed for definitive analyses of the molecular characteristics and cellular behavior of LTB/sub 4/ receptors.

• Research Organization: Univ. of California Medical Center, San Francisco
• OSTI ID: 5325822
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ARACHIDONIC ACID
BIOCHEMICAL REACTION KINETICS
RECEPTORS
NEUTROPHILS
MOLECULAR BIOLOGY
AFFINITY
ANTIBODIES
CELL MEMBRANES
MICE
MONOCLONAL ANTIBODIES
RABBITS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques