Fatty acid profiles of mammary gland tissue in rats following exposure to 7,12-dimethylbenz(a)anthracene
Prostaglandins have been implicated in the mechanism of DMBA carcinogenesis and tumor-promoting effects of linoleic acid (C18:2 omega 6). Since elevated levels of arachidonate (C20:4 omega 6) products have been reported in various mammary tumors it is important to evaluate changes in pools of this substrate fatty acid and its precursor, linoleic acid. To address this question, total lipid extracts were subjected to TLC separation to obtain phosphatidyl choline (PC), phosphatidyl ethanolamine (PE), and neutral (N) fractions. Fatty acid profiles for each fraction were analyzed by GLC. Three groups of 50 day old female Sprague-Dawley rats were used: Control, DMBA 10mg and 20mg/rat. Mammary gland tissue specimens were excised at 2 week intervals from the time of challenge through appearance of tumors. Individual fatty acid percentages and specific ratios were examined. At 2 weeks post DMBA, the levels of C18:2 omega 6 were increased in all 3 lipid fractions in both DMBA-challenged compared with Control groups. On the other hand, similar trends in C20:4 omega 6 were apparent only in the PE fraction. Also, ratio of 20:4/18:2 showed a marked increase from the Control (0.09) to DMBA-challenged groups (0.47 and 0.42 respectively) in the PE fraction. These results suggest changes in fatty acid profiles during the early phase of DMBA carcinogenesis. Results of similar studies at subsequent stages will also be discussed in detail.
- Research Organization:
- Rutgers, The State Univ. of New Jersey, New Brunswick
- OSTI ID:
- 5324591
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DIMETHYLBENZANTHRACENE
CARCINOGENESIS
MAMMARY GLANDS
NEOPLASMS
ONCOGENIC TRANSFORMATIONS
BIOCHEMISTRY
PHOSPHOLIPIDS
METABOLISM
ARACHIDONIC ACID
GAS CHROMATOGRAPHY
LINOLEIC ACID
RATS
THIN-LAYER CHROMATOGRAPHY
ANIMALS
AROMATICS
BODY
CARBOXYLIC ACIDS
CELL TRANSFORMATIONS
CHEMISTRY
CHROMATOGRAPHY
CONDENSED AROMATICS
DISEASES
ESTERS
GLANDS
LIPIDS
MAMMALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PATHOGENESIS
RODENTS
SEPARATION PROCESSES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)